IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOM20 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit TOM20 Polyclonal Antibody | anti-TOM20 antibody
TOM20 Rabbit pAb
IHC: 1:50-1:200
IF: 1:50-1:200
Customer Validation:
WB: Mouse, Human
IF: Human
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOM20 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOM20 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human colon using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat kidney using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat kidney using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse kidney using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse kidney using TOM20 Rabbit pAb at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using TOM20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using TOM20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5min.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5min.)
NCBI and Uniprot Product Information
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Product Notes
The TOM20 tomm20 (Catalog #AAA28341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOM20 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TOM20 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 Customer Validation: WB: Mouse, Human IF: Human. Researchers should empirically determine the suitability of the TOM20 tomm20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOM20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is TOM20 used as a marker for in mitochondrial studies?
TOM20 is used as a marker for mitochondrial studies, specifically the outer mitochondrial membrane. Researchers use it to detect where the mitochondria are inside the cells and to study their number, shape, and distribution during normal conditions or disease-related changes.
Can a TOM20 antibody be used to visualize mitochondria by immunofluorescence?
Yes, TOM20 antibodies help visualize mitochondria in immunofluorescence experiments. Fluorescent labels make their shape, size, and position stand out clearly under a microscope.
What is the difference between TOM20 and other mitochondrial markers?
TOM20 marks just the outer membrane of mitochondria. Other markers target the inner membrane, the inner matrix, or mitochondrial functions, such as metabolism. So, TOM20 is great for studying structure, not activity or enzymes.
Is TOM20 expression affected by mitochondrial stress or damage?
Yes, TOM20 levels can change during mitochondrial stress or damage. In TOM20, expression can drop, or its pattern can change in certain conditions. Researchers use it to track mitochondrial health and detect structural shifts resulting from stress or disease.
Can TOM20 antibody be used for co-localization with other mitochondrial proteins?
Yes, the TOM20 antibody can be used for co-localization studies. Researchers stain TOM20 with other mitochondrial proteins to verify that proteins are inside mitochondria and to examine how components interact or shift positions.
Does fixation method affect TOM20 staining quality?
Yes, the fixation method strongly affects TOM20 staining. Paraformaldehyde preserves TOM20 structure effectively, while harsh fixatives reduce signal quality. Proper fixation maintains mitochondrial shape and ensures consistent TOM20 staining under the microscope.
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