IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.)
anti-Human, Mouse B-RAF Antibody | anti-B-RAF antibody
B-RAF (Phospho-Ser446) Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
B-RAF; N/A; B-RAF (Phospho-Ser446) Antibody; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; EC 2.7.1.37; EC 2.7.11.1; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1; anti-B-RAF antibody
Reactivity
Human, Mouse
Specificity
B-RAF (Phospho-Ser446) antibody detects endogenous levels of B-RAF only when phosphorylated at serine 446.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
766
Applicable Applications for anti-B-RAF antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).
All Sites
Human: Ser446
Mouse: Ser483
Mouse: Ser483
Preparation and Storage
Store at-20 degree C for 1 year.
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-B-RAF antibody
References
Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992). Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases. Hillier L.W., Nature 424:157-164(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,437 Da
NCBI Official Full Name
serine/threonine-protein kinase B-raf isoform 1
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; B-raf; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
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Product Notes
The B-RAF braf (Catalog #AAA110765) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The B-RAF (Phospho-Ser446) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B-RAF can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the B-RAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-RAF, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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