Application Data
(3.3 uM H3K36me2 peptide was incubated with 100 nM FBXL11 / KDM2A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K36me2 peptide was used as negative control.)
KDM2A/FBXL11 recombinant protein
Recombinant KDM2A/FBXL11 protein
Gene Names
KDM2A; FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA
Purity
The recombinant protein is >75% pure by SDS-PAGE.
Synonyms
KDM2A/FBXL11; N/A; Recombinant KDM2A/FBXL11 protein; Lysine Demethylase 2A; Jumonji C Domain-Containing Histone Demethylase 1A; JHDM1A; KDM2A/FBXL11 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >75% pure by SDS-PAGE.
Form/Format
Full length recombinant FBXL11/KDM2A protein was expressed in Sf9 cells and is supplied in 25mM HEPES pH7.5, 500mM NaCl, 10% glycerol, and 0.5mM TCEP.
Concentration
0.4 ug/mL (varies by lot)
Source
Human
Specific Activity
H3K36me2 demethylase.
Expressed In
Baculovirus
Catalytic Ability
13 turnovers/ enzyme molecule.
Histone Demethylase Assay Conditions
3.3 uM H3K36me2 peptide was incubated with 100 nM recombinant FBXL11 / KDM2A protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 uM 2OG, 100 uM Ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 2 hours at room temperature. MALDI-TOF was used for detection
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(3.3 uM H3K36me2 peptide was incubated with 100 nM FBXL11 / KDM2A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K36me2 peptide was used as negative control.)
Application Data
(3.3 uM H3K36me2 peptide was incubated with 100 nM FBXL11 / KDM2A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K36me2 peptide was used as negative control.)
SDS-PAGE
(Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
SDS-PAGE
(Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
Related Product Information for KDM2A/FBXL11 recombinant protein
Short Description: Recombinant KDM2A/FBXL11 (accession number NP_036440.1) was expressed in Sf9 and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 128 kDa. The recombinant protein is >75% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: Lysine (K)-specific demethylase 2A (KDM2A), also known as FBXL11, JHDM1A, CXXC8, nucleates at CpG islands and specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
Background: Lysine (K)-specific demethylase 2A (KDM2A), also known as FBXL11, JHDM1A, CXXC8, nucleates at CpG islands and specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
Product Categories/Family for KDM2A/FBXL11 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128 kDa
NCBI Official Full Name
lysine-specific demethylase 2A isoform a
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 2A
NCBI Official Symbol
KDM2A
NCBI Official Synonym Symbols
FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA
NCBI Protein Information
lysine-specific demethylase 2A; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-rich repeat protein 11; jumonji C domain-containing histone demethylase 1A; jmjC domain-containing histo
UniProt Protein Name
Lysine-specific demethylase 2A
UniProt Gene Name
KDM2A
UniProt Synonym Gene Names
CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004
UniProt Entry Name
KDM2A_HUMAN
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Product Notes
The KDM2A/FBXL11 kdm2a (Catalog #AAA59713) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KDM2A/FBXL11, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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