Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ?g/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml.)
Lymphotoxin-alpha (LTA) Active Protein | LTA active protein
Recombinant Human Lymphotoxin-alpha (LTA)(Active)
Gene Names
LTA; LT; TNFB; TNFSF1
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Lymphotoxin-alpha (LTA); N/A; Recombinant Human Lymphotoxin-alpha (LTA)(Active); LTA active protein
Host
Mammalian cell
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Sequence Positions
35-205aa; Full Length of Mature Protein
Sequence
LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL$
Species
Homo sapiens (Human)
Tag
N-terminal 6xHis-tagged
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Research Area
Cytokine
Biological Activity
1. Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ug/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ug/ml can bind human TNFR1, the EC50 of human LTA protein is 4.409-6.797 ng/ml.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ?g/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml.)
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 ?g/ml can bind human TNFRSF1B, the EC50 is 1.632-2.699 ng/ml.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Product Categories/Family for LTA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,297 Da
NCBI Official Full Name
lymphotoxin alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1
NCBI Protein Information
lymphotoxin-alpha; LT-alpha; TNF-beta; TNF superfamily, member 1; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
UniProt Entry Name
TNFB_HUMAN
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Product Notes
The LTA lta (Catalog #AAA279218) is an Active Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 35-205aa; Full Length of Mature Protein. The amino acid sequence is listed below: LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L$. It is sometimes possible for the material contained within the vial of "Lymphotoxin-alpha (LTA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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