WB (Western Blot)
(Western blot analysis of extracts of human spleen, using LTA antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit LTA Polyclonal Antibody | anti-LTA antibody
LTA Antibody
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Synonyms
LTA, Antibody; LTA Antibody; LTalpha; DIF; LT alpha; LT; LT-alpha; Lta; Lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxin-alpha; TNF B; TNF superfamily member 1; TNF, lymphocyte-derived; TNF-beta; TNFB; TNFB_HUMAN; TNFbeta; TNFSF1; TNLG1E; Tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1; anti-LTA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
205
Applicable Applications for anti-LTA antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TNF beta, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of human spleen, using LTA antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts of human spleen, using LTA antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA326222 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA326222 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemistry)
(AAA326222 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemistry)
(AAA326222 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Related Product Information for anti-LTA antibody
Description: Lymphotoxin-alpha (LT-alpha), also known as TNF-beta, is a member of the TNF superfamily of proteins. NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-alpha. LT-alpha can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-beta (LT alpha1beta2 or LT alpha2beta1) which can be cleaved from the cell surface by matrix metalloproteases!!!!Soluble LT-alpha binds to and signals through TNFR1/TNFR2, activating the canonical NF-kappaB pathway 2. In contrast, LT alpha1beta2 heterodimers bind to the LTbetaR receptor and activate the noncanonical NF-kappaB pathway. As a result, LT-alpha and TNF-alpha have overlapping functions. Soluble LT-alpha and LT alpha1beta2 play key roles in lymphangiogenesis. The LT alpha1beta2/LTbetaR axis is essential for the development of lymphoid tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22 kDa
NCBI Official Full Name
lymphotoxin-alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1; TNLG1E
NCBI Protein Information
lymphotoxin-alpha
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
Similar Products
Product Notes
The LTA lta (Catalog #AAA326222) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LTA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LTA can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LTA lta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LTA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.