Mouse Midkine ELISA Kit | MDK elisa kit

Mouse Midkine PicoKine ELISA Kit

Cat. Num. AAA125228

• Gene Names: Mdk; MK; Mek
• Reactivity: Mouse
• Synonyms: Midkine; N/A; Mouse Midkine PicoKine ELISA Kit; Mouse Midkine; MK; Retinoic acid-induced differentiation factor; Mdk; Mk; MDK elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Mdk
• Sequence Length: 140
• Samples: Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for MDK elisa kit

Background: Midkine (MK) is a member of a family of neurotrophic and developmentally-regulated heparin-binding molecules that currently consists of midkine, pleiotrophin/PTN and avian RI-HB. This gene is mapped to 2 E1; 2 50.63 cM. This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants.

Principle of the Assay: The Picokine™ Mouse Mdk Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Mdk with a 96-well strip plate that is pre-coated with antibody specific for Mdk. The detection antibody is a biotinylated antibody specific for Mdk. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Mdk protein as standards. To measure Mouse Mdk, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Mdk in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Mdk in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 59894790
• NCBI GeneID: 17242
• NCBI Accession #: NP_001012335.1
• NCBI GenBank Nucleotide #: NP_001012335.1
• UniProt Accession #: P12025
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: Mdk
• NCBI Official Synonym Symbols: MK; Mek
• NCBI Protein Information: midkine
• UniProt Protein Name: Midkine
• UniProt Gene Name: Mdk
• UniProt Synonym Gene Names: Mk; MK
• UniProt Entry Name: MK_MOUSE