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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MDK Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, mouse MDK Polyclonal Antibody | anti-MDK antibody
MDK Antibody (C-term)
Gene Names
MDK; MK; ARAP; NEGF2
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MDK, Antibody; MDK Antibody (C-term); Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK; MK1; NEGF2; anti-MDK antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the C-terminal region of human MDK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
113-143
Sequence Length
87
Applicable Applications for anti-MDK antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MDK Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MDK Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MDK Antibody (C-term) with Hela cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MDK Antibody (C-term) with Hela cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MDK Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).)
WB (Western Blot)
(MDK Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).)
Related Product Information for anti-MDK antibody
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).
Product Categories/Family for anti-MDK antibody
References
Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010) Kerzerho, J., et al. J. Immunol. 185(1):418-423(2010) Yao, X., et al. Acta Pharmacol. Sin. 31(5):629-637(2010) Zhang, Z.H., et al. Cell Prolif. 43(2):184-194(2010) Rice, G.E., et al. J. Exp. Clin. Cancer Res. 29, 62 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15585
NCBI Official Full Name
midkine isoform a
NCBI Official Synonym Full Names
midkine (neurite growth-promoting factor 2)
NCBI Official Symbol
MDK
NCBI Official Synonym Symbols
MK; ARAP; NEGF2
NCBI Protein Information
midkine
UniProt Protein Name
Midkine
UniProt Gene Name
MDK
UniProt Synonym Gene Names
MK1; NEGF2; MK; ARAP
UniProt Entry Name
MK_HUMAN
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Product Notes
The MDK mdk (Catalog #AAA290412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 113-143. The MDK Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MDK can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MDK mdk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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