SDS-PAGE
(Figure 1. Human MDK Protein, hFc Tag on SDS-PAGE under reducing condition.)
MDK recombinant protein
Human MDK Protein, hFc Tag
Gene Names
MDK; MK; ARAP; NEGF2
Purity
>95.0% as determined by SDS-PAGE and Coomassie blue staining.Synonyms
MDK; N/A; Human MDK Protein, hFc Tag; Midkine; MK; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK recombinant protein
Host
HEK293
Purity/Purification
>95.0% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Concentration
0.95mg/mL (varies by lot)
Tag
C-Human Fc tag
Molecular Characterization
MDK(Val21-Asp143) hFc(Glu99-Ala330)
Molecular Weight Note
The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide. The apparent molecular mass of MDK-hFc is approximately 35-55 kDa due to glycosylation.
Reconstitution
1: Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.
2: Dissolved lyophilized protein sample in sterile water based on the recommended volume 105.26ul.
3:After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
2: Dissolved lyophilized protein sample in sterile water based on the recommended volume 105.26ul.
3:After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
Visual Inspection
Test: Lyophilization ; Specification: Dried white powder; Result: Pass
Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass
Test: Bottle Sealing (Mannual check) ; Specification: Sealed tight ; Result: Pass
Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass
Test: Bottle Sealing (Mannual check) ; Specification: Sealed tight ; Result: Pass
Quantity
Test: Bradford Method ; Specification: >specified quantity ; Result: Pass
Other properties
Test: pH ; Specification: 7.4 ± 0.5 ; Result: Pass
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
SDS-PAGE
(Figure 1. Human MDK Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human MDK Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for MDK recombinant protein
Recombinant human MDK protein with C-terminal human Fc tag
This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]
This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]
Product Categories/Family for MDK recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
midkine isoform a
NCBI Official Synonym Full Names
midkine (neurite growth-promoting factor 2)
NCBI Official Symbol
MDK
NCBI Official Synonym Symbols
MK; ARAP; NEGF2
NCBI Protein Information
midkine; retinoic acid inducible factor; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting factor 2
UniProt Protein Name
Midkine
UniProt Gene Name
MDK
UniProt Synonym Gene Names
MK1; NEGF2; MK; ARAP
UniProt Entry Name
MK_HUMAN
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Product Notes
The MDK mdk (Catalog #AAA47549) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MDK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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