Human MMP-1 ELISA Kit | MMP1 elisa kit
Human MMP-1 PicoKine ELISA Kit
Gene Names
MMP1; CLG; CLGN
Reactivity
Human
Synonyms
MMP-1; N/A; Human MMP-1 PicoKine ELISA Kit; Interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); 27 kDa interstitial collagenase; CLG; CLGN; Fibroblast collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP1; MMP1_HUMAN; MMP1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human MMP1
Sequence Length
403
Samples
Cell culture supernatants, serum and plasma (heparin)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<20 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Related Product Information for MMP1 elisa kit
Background: Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smokinginduced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Principle of the Assay: The Picokine™ Human MMP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human MMP1 with a 96-well strip plate that is pre-coated with antibody specific for MMP1. The detection antibody is a biotinylated antibody specific for MMP1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MMP1 with immunogen: Expression system for standard: NS0; Immunogen sequence: F20-N469. The kit is analytically validated with ready-to-use reagents. To measure Human MMP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human MMP1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MMP1 in the sample.
Principle of the Assay: The Picokine™ Human MMP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human MMP1 with a 96-well strip plate that is pre-coated with antibody specific for MMP1. The detection antibody is a biotinylated antibody specific for MMP1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MMP1 with immunogen: Expression system for standard: NS0; Immunogen sequence: F20-N469. The kit is analytically validated with ready-to-use reagents. To measure Human MMP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human MMP1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MMP1 in the sample.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
46,266 Da
NCBI Official Synonym Full Names
matrix metallopeptidase 1 (interstitial collagenase)
NCBI Official Symbol
MMP1
NCBI Official Synonym Symbols
CLG; CLGN
NCBI Protein Information
interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1
UniProt Protein Name
cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7)
UniProt Entry Name
B4DN15_HUMAN
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Product Notes
The Human MMP1 (Catalog #AAA11553) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11553 ELISA Kit recognizes Human MMP1. It is sometimes possible for the material contained within the vial of "MMP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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