Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Detection limit for recombinant GST tagged PLXNA2 is 0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of PLXNA2 expression in transfected 293T cell line by PLXNA2 monoclonal antibody. Lane 1: PLXNA2 transfected lysate (Predicted MW: 18.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in NIH/3T3.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in Raw 264.7.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in HepG2)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (43.78kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M04), clone 5B11 Western Blot analysis of TIMP2 expression in HeLa.)
WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CSTF3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (37.07kD).)
WB (Western Blot) (SF3B2 monoclonal antibody. Western Blot analysis of SF3B2 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SF3B2 on HeLa cell. [antibody concentration 60ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SF3B2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml].)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in NIH/3T3.)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (32.05kD).)
Application Data (Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 40ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody. Lane 1: MPG transfected lysate (32.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MPG monoclonal antibody Western Blot analysis of MPG expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Chk1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining Chk1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Chk1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Chk1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Chk1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Chk1 on different lysates using anti-Chk1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-3M)
SDS-PAGE (Raji whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD79a(ABT-CD79a) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (Western Blot analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody. Lane 1: CTNNB1 transfected lysate (85.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with FLT1 rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with GSK3B rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CTNNB1 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CTNNB1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MRPL12 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody. Lane 1: MRPL12 transfected lysate (21.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MRPL12 monoclonal antibody, Western Blot analysis of MRPL12 expression in COLO 320 HSR.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in PC-12.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1 Western Blot analysis of AKT2 expression in Jurkat.)
FCM (Flow Cytometry) (Overlay histogram showing HepG2 cells stained with AAA28061 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA28061 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing A549 cells stained with AAA28061 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells with AAA28061 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA28061 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with AAA28061 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistochemistry) (IHC image of AAA28061 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA28061 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA28061 diluted at 1:500 and staining in paraffin-embedded human glioma tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: Raji whole cell lysateAll lanes CD63 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 30-120 KD KDaObserved band size: 30-120 KD KDaExposure time?1min)
WB (Western Blot) (Western BlotPositive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysateAll lanes CD63 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 30-120 KD KDaObserved band size: 30-120 KD KDaExposure time?1min)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)
WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)
WB (Western Blot) (CBS monoclonal antibody. Western Blot analysis of CBS expression in MCF-7.)
WB (Western Blot) (CBS monoclonal antibody, Western Blot analysis of CBS expression in HeLa.)
WB (Western Blot) (Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 124401. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CBS is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CBS transfected lysate using 124401and Protein A Magnetic Bead and immunoblotted with CBS rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of formalin-fixed paraffin-embedded human hepatocellular carcinoma using 124401 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of CBS expression in transfected 293T cell line by 124401. Lane 1: CBS transfected lysate (61kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)
Application Data (Detection limit for recombinant GST tagged RAB7B is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of RAB7B transfected lysate using RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody. Lane 1: RAB7B transfected lysate (22.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in human kidney.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with PFKFB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining PFKFB3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: A431)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 3(ABT-CLD3) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (Western blot analysis of Claudin 3Antibody at 1:1000 dilution.)
WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)
WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)
WB (Western Blot) (CCNH monoclonal antibody, Western Blot analysis of CCNH expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CCNH is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody. Lane 1: CCNH transfected lysate (37.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)
WB (Western Blot) (ACBD3 monoclonal antibody, Western Blot analysis of ACBD3 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged ACBD3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACBD3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody. Lane 1: ACBD3 transfected lysate (60.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G
Gene Names
ACBD3; PAP7; GCP60; GOCAP1; GOLPH1
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)
WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)
Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)
Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)
Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in NIH/3T3.)
WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in Raw 264.7.)
WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in Hela NE.)
WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with C14orf93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining C14orf93 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate (66.4 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat (Cat # L017V1).)
Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)
Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)
Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1, 000 dilution. Positive control�� Lane1: Rat kidney Lane2: PC-12 Lane3: Hela Lane4: Mouse spleen Lane5: NIH-3T3 Lane6: Rat spleen)
Application Data (Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Application Data (Overlay histogram showing U251 cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescent analysis of U251 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence) (Immunofluorescent analysis of PC-3 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human melanoma using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney tissue using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA18857 at dilution of 1:100.)
WB (Western Blot) (Western Blot Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate All lanes: NES antibody at 3 ug/mlPredicted band size: 260 kDa Observed band size: 260 kDa )
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
Application Data (Detection limit for recombinant GST tagged CAMKK2 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CAMKK2 transfected lysate using CAMKK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CAMKK2 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of CAMKK2 expression in transfected 293T cell line by CAMKK2 monoclonal antibody Lane 1: CAMKK2 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in K-562.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in IMR-32)
WB (Western Blot) (Western Blot detection against Immunogen (40.3kD).)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)
WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)
Application Data (Detection limit for recombinant GST tagged WWP1 is 0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of WWP1 transfected lysate using WWP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with WWP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody Lane 1: WWP1 transfected lysate (105.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
IP (Immunoprecipitation) (Immunoprecipitation of PSMA7 transfected lysate using PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PSMA7 monoclonal antibody Western Blot analysis of PSMA7 expression in HeLa.)
WB (Western Blot) (PSMA7 monoclonal antibody. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in PC-12.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1 Western Blot analysis of AKT2 expression in Jurkat (Cat # L017V1).)
IP (Immunoprecipitation) (Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (2ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 )
FCM (Flow Cytometry) (Overlay histogram showing 293 cells transfected with PD-L2 stained with (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (2ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of U251 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Raji cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug All lanes: PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
WB (Western Blot) (Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus muscle tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded hman stamoch tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1, 000 dilution.)
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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