Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
WB (Western Blot) (Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody Lane 1: VRK2 transfected lysate (58.1kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged VRK2 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (VRK2 monoclonal antibody, Western Blot analysis of VRK2 expression in K-562.)
WB (Western Blot) (VRK2 monoclonal antibody. Western Blot analysis of VRK2 expression in U-2 OS.)
WB (Western Blot) (Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged POU4F3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody. Lane 1: POU4F3 transfected lysate (37.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (POU4F3 monoclonal antibody Western Blot analysis of POU4F3 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.75kD).)
WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody. Lane 1: NEK10 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate (105.4 KDa).Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control�� Line 1: MCF-7 Line 2: A549)
WB (Western Blot) (SMAD1 monoclonal antibody Western Blot analysis of SMAD1 expression in A-549.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Detection limit for recombinant GST tagged SMAD1 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody. Lane 1: SMAD1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SMAD1 monoclonal antibody. Western Blot analysis of SMAD1 expression in MES-SA/Dx5.)
WB (Western Blot) (Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NDN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5ug/ml])
WB (Western Blot) (Western Blot analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody. Lane 1: NDN transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDN monoclonal antibody Western Blot analysis of NDN expression in HL-60.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.)
IP (Immunoprecipitation) (Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GABPA monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
SDS-PAGE (K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Perforin(ABT355)antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistochemistry) (Human spleen tissue was stained with Anti-Perforin (ABT355) Antibody)
IHC (Immmunohistochemistry) (Human spleen tissue was stained with Anti-Perforin (ABT355) Antibody)
IHC (Immunohistochemistry) (Human spleen tissue was stained with Anti-Perforin (ABT355) Antibody)
IHC (Immunohistochemistry) (Human spleen tissue was stained with Anti-Perforin (ABT355) Antibody)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE)
Application Data (Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)
IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PML is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in Hela NE.)
WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
WB (Western Blot) (Western blot analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NDUFS4 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody. Lane 1: NDUFS4 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDUFS4 monoclonal antibody Western Blot analysis of NDUFS4 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug Bax antibody (AAA28539). Western blot was performed from the immunoprecipitate using Bax antibody (AAA28539) at a dilution of 1:500.)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Bax Rabbit mAb (AAA28539) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Bax Rabbit mAb (AAA28539) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Bax Rabbit mAb (AAA28539) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit mAb (AAA28539) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot) (Western blot analysis of various lysates, using [KO Validated] Bax Rabbit mAb (AAA28539) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with ASK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ASK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASK1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining TDP43 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TDP43 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TDP43 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TDP43 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP43 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TDP43 on different cells lysates using anti-TDP43 antibody at 1/500 dilution. Positive control�� Line 1: Hela Line 2: K563)
WB (Western Blot) (G6PD Antibody-Western blot using G6PD monoclonal antibody against human G6PD (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa))
WB (Western Blot) (G6PD Antibody-Western blot using G6PD mouse monoclonal antibody against HeLa (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate.)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human pancreas tissue using Somatostatin (SST) Rabbit mAb (AAA28543, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Somatostatin (SST) Rabbit mAb (AAA28543) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Somatostatin (SST) Rabbit mAb (AAA28543) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Somatostatin (SST) Rabbit mAb (AAA28543) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Somatostatin (SST) Rabbit mAb (AAA28543) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Somatostatin (SST) Rabbit mAb (AAA28543) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with TTR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining TTR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TTR in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TTR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-TTR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- TTR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TTR antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution. Positive control�� Line 1: Human brain Line 2��Human lung)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged P15RS is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to P15RS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (P15RS monoclonal antibody, Western Blot analysis of P15RS expression in A-431.)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.56kD).)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)
IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)
WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.7kD).)
WB (Western Blot) (Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LCK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LCK transfected lysate using LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody. Lane 1: LCK transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LCK monoclonal antibody Western Blot analysis of LCK expression in Jurkat.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)
WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12 (Cat # L012V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NFAT1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining NFAT1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NFAT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NFAT1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
WB (Western Blot) (CAMKK1 monoclonal antibody, Western Blot analysis of CAMKK1 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged CAMKK1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody. Lane 1: CAMKK1 transfected lysate (57.31kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (83.31kD).)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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