Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (GABPA monoclonal antibody (M07), clone 5C8 Western Blot analysis of GABPA expression in Hela S3 NE (Cat # L013V3).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with 53BP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining 53BP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 53BP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 53BP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-53BP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-53BP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-53BP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-53BP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-53BP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)
WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)
WB (Western Blot) (Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RAD51C is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody. Lane 1: RAD51C transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RAD51C monoclonal antibody. Western Blot analysis of RAD51C expression in 293.)
WB (Western Blot) (RAD51C monoclonal antibody, Western Blot analysis of RAD51C expression in HeLa.)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody. Lane 1: LETM1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562 (Cat # L009V1).)
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
ICC (Immunocytochemistry) (ICC staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1, 000 dilution. Positive control: Lane 1: PC12 Lane 2: 293T)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
WB (Western Blot) (KHDRBS1 monoclonal antibody Western Blot analysis of KHDRBS1 expression in A-431)
Application Data (Detection limit for recombinant GST tagged KHDRBS1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KHDRBS1 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody. Lane 1: KHDRBS1 transfected lysate (41kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse lung using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TOMM20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12)
Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)
WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)
WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
SDS-PAGE (Whole cell lysates of HEK293 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-LIMK-1/2(PTR2545) antibody. The HRP-conjugated Goat anti-M)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37 degree C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Actin(ABT-ACTN) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (Western blot analysis of Actin-panAntibody at 1:1000 dilution.)
Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)
WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (AKR1B10 monoclonal antibody, Western Blot analysis of AKR1B10 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged AKR1B10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKR1B10 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody. Lane 1: AKR1B10 transfected lysate (36kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (33.59kD).)
WB (Western Blot) (TGIF2 monoclonal antibody, Western Blot analysis of TGIF2 expression in HeLa NE.)
WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in SW-13.)
WB (Western Blot) (Western blot analysis of TGIF2 over-expressed 293 cell line, cotransfected with TGIF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGIF2 monoclonal antibody (M01). GAPDH (36.1kD). used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TGIF2 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of TGIF2 expression in transfected 293T cell line by TGIF2 monoclonal antibody. Lane 1: TGIF2 transfected lysate (25.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat (Cat # L017V1).)
WB (Western Blot) (Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M03), clone 1B11.Lane 1: HMGB1 transfected lysate (25 KDa).Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged HMGB1 is approximately 3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA27026 (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells with AAA27026 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA27026 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistochemistry) (IHC image of AAA27026 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27026 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA27026 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A/C antibody. Western blot was performed from the immunoprecipitate using Lamin A/C antibody at a dilition of 1:20000.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
WB (Western Blot) (LZTFL1 monoclonal antibody, Western Blot analysis of LZTFL1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoDetection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.clonal antibody to LZTFL1 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LZTFL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of LZTFL1 expression in transfected 293T cell line by LZTFL1 monoclonal antibody. Lane 1: LZTFL1 transfected lysate (34.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed U87MG cells using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)
WB (Western Blot) (Western Blot Analysis of HEK293 cell lysate using RCC Mouse Monoclonal Antibody (CA9/3406).)
SDS-PAGE (SDS-PAGE Analysis Purified CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Confirmation of Integrity and Purity of Antibody.)
IHC (Immunohistochemistry-Formalin) (Formalin-fixed, paraffin-embedded human Kidney stained with CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406).)
IHC (Immunohistochemistry-Formalin) (Formalin-fixed, paraffin-embedded human Kidney stained with CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406).)
IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed paraffin-embedded human tonsil using 1267661 (3ug/ml) and Immunoperoxidase.)
IP (Immunoprecipitation) (Immunoprecipitation of FGF1 transfected lysate using 126766 and Protein A Magnetic Bead and immunoblotted with FGF1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of FGF1 expression in transfected 293T cell line using 126766. Lane 1: transfected lysate (17.5kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged FGF1 is ~10ng/ml using 126766 as a capture antibody.)
WB (Western Blot) (AKAP10 monoclonal antibody Western Blot analysis of AKAP10 expression in Raw 264.7.)
WB (Western Blot) (RNAi Knockdown: Western blot analysis of AKAP10-over-expressing 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with Cat. # 123131. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged AKAP10 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase staining of AKAP10 on formalin-fixed paraffin-embedded human testis using Cat. # 123131 at 0.5ug/ml.)
WB (Western Blot) (Western Blot analysis of AKAP10 expression in transfected 293T cell line using Cat. # 123131: Lane 1: AKAP10 transfected lysate (73.8kD); Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
AKAP10 (A-kinase Anchor Protein 10, Mitochondrial, AKAP-10, Dual Specificity A Kinase-anchoring Protein 2, D-AKAP-2, Protein Kinase A-anchoring Protein 10, PRKA10) (Biotin)
Gene Names
AKAP10; PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
Reactivity
Human, Mouse
Applications
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
WB (Western Blot) (Western Blot detection against Immunogen (54.71kD).)
WB (Western Blot) (TARDBP monoclonal antibody Western Blot analysis of TARDBP expression in A-431.)
WB (Western Blot) (Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TARDBP is 0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of TARDBP transfected lysate using TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody. Lane 1: TARDBP transfected lysate (44.7kD). Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ORM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ORM1 transfected lysate using ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody. Lane 1: ORM1 transfected lysate (23.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ORM1 monoclonal antibody Western Blot analysis of ORM1 expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (45.98kD).)
WB (Western Blot) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody. Lane 1: PCDH8 transfected lysate (113kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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