ICC (Immunocytochemistry)
(Confocal imaging of RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
Rabbit anti-Human ACC1/ACC2-S79 Monoclonal Antibody | anti-ACACA/ACACB antibody
Phospho-ACC1/ACC2-S79 Rabbit PolymAb
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
ACC1/ACC2-S79, Antibody; Phospho-ACC1/ACC2-S79 Rabbit PolymAb; ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha; Phospho-Acetyl CoA Carboxylase-S79; anti-ACACA/ACACB antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
SSMSG
Applicable Applications for anti-ACACA/ACACB antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S79 of human Acetyl CoA Carboxylase.
Modification
Phospho S79
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
ICC (Immunocytochemistry)
(Confocal imaging of RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
WB (Western Blot)
(Western blot analysis of lysates from RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AAA283153) at 1:2000 dilution incubated overnight at 4?. RAW 264.7 cells were treated by oligomycin (0.5 uM) at 37? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AAA283153) at 1:2000 dilution incubated overnight at 4?. RAW 264.7 cells were treated by oligomycin (0.5 uM) at 37? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AAA283153) at 1:2000 dilution incubated overnight at 4?. A431 cells were treated by ?-PP mixed solution (1ul) at 30? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AAA283153) at 1:2000 dilution incubated overnight at 4?. A431 cells were treated by ?-PP mixed solution (1ul) at 30? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-ACACA/ACACB antibody
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Product Categories/Family for anti-ACACA/ACACB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 265kDa
Observed MW: 266kDa
Observed MW: 266kDa
UniProt Protein Name
Acetyl-CoA carboxylase 1
UniProt Gene Name
ACACA
UniProt Synonym Gene Names
ACAC; ACC1; ACCA; ACC1
UniProt Entry Name
ACACA_HUMAN
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Product Notes
The ACACA/ACACB acaca (Catalog #AAA283153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ACC1/ACC2-S79 Rabbit PolymAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACC1/ACC2-S79 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ACACA/ACACB acaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SSMSG. It is sometimes possible for the material contained within the vial of "ACC1/ACC2-S79, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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