WB (Western Blot)
(Western blot analysis of Phospho-ACC1 (Thr1196) in lysates of 293, using Phospho-ACC1 (Thr1196) Antibody.)
Rabbit ACC1 Polyclonal Antibody | anti-ACC1 antibody
Phospho-ACC1 (Thr1196) Antibody
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Human, Mouse, RatPredicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
ACC1, Antibody; Phospho-ACC1 (Thr1196) Antibody; ACAC; ACACA; ACACA_HUMAN; ACACB; ACC alpha; ACC; ACC beta; ACC-alpha; ACC1; ACC2; ACCA; ACCB; Acetyl CoA carboxylase 1; Acetyl CoA carboxylase 2; Acetyl CoA carboxylase alpha; Acetyl CoA carboxylase beta; Acetyl Coenzyme A carboxylase alpha; Acetyl Coenzyme A carboxylase beta; Biotin carboxylase; COA1; COA2; HACC275; OTTHUMP00000164069; OTTHUMP00000164070; OTTHUMP00000164076; OTTHUMP00000240532; anti-ACC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-ACC1 (Thr1196) Antibody detects endogenous levels of ACC1 only when phosphorylated at Thr1196.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2383
Applicable Applications for anti-ACC1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ACC1 around the phosphorylation site of Thr1196.
Tissue Specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-ACC1 (Thr1196) in lysates of 293, using Phospho-ACC1 (Thr1196) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-ACC1 (Thr1196) in lysates of 293, using Phospho-ACC1 (Thr1196) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining human gastric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining human gastric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-ACC1 antibody
Description: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Post Translational Modifications: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Subunit Structure: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
Post Translational Modifications: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Subunit Structure: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 280 kDa
Predicted: 266 kDa
Predicted: 266 kDa
NCBI Official Full Name
acetyl-CoA carboxylase 1 isoform 1
NCBI Official Synonym Full Names
acetyl-CoA carboxylase alpha
NCBI Official Symbol
ACACA
NCBI Official Synonym Symbols
ACC; ACAC; ACC1; ACCA; ACACAD
NCBI Protein Information
acetyl-CoA carboxylase 1
UniProt Protein Name
Acetyl-CoA carboxylase 1
UniProt Gene Name
ACACA
UniProt Synonym Gene Names
ACAC; ACC1; ACCA; ACC1
UniProt Entry Name
ACACA_HUMAN
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Product Notes
The ACC1 acaca (Catalog #AAA328495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ACC1 (Thr1196) Antibody reacts with Human, Mouse, Rat Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ACC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ACC1 acaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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