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product-image-AAA17777_SDS_PAGE2.jpg SDS-PAGE (human heart tissue were subjected to SDS PAGE followed by western blot with AAA17777 (Actin cardiac actin-Specific antibody) at dilution of 1:5000)

Mouse Alpha cardiac actin Monoclonal Antibody

anti- Alpha cardiac actin-specific antibody

Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purification: Protein A+G purification
Purity: >95% as determined by SDS-PAGE
Synonyms
Alpha cardiac actin, Antibody; anti- Alpha cardiac actin-specific antibody; ACTC; ACTC1; ACTC1 cardiac muscle 1; ACTC1 specific; Actin cardiac muscle specific; Actin; alpha cardiac muscle 1; actin; alpha; cardiac muscle 1; Alpha cardiac actin; CMD1R; CMH11.; anti-Alpha cardiac actin antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A1
Purity/Purification
Purification: Protein A+G purification
Purity: >95% as determined by SDS-PAGE
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Applicable Applications for anti-Alpha cardiac actin antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:2000-1:10000
IHC: 1:100-1:400
IF: 1:50-1:500
Immunogen
actin, alpha, cardiac muscle 1
Preparation and Storage
Store at -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

SDS-PAGE

(human heart tissue were subjected to SDS PAGE followed by western blot with AAA17777 (Actin cardiac actin-Specific antibody) at dilution of 1:5000)

product-image-AAA17777_SDS_PAGE2.jpg SDS-PAGE (human heart tissue were subjected to SDS PAGE followed by western blot with AAA17777 (Actin cardiac actin-Specific antibody) at dilution of 1:5000)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human heart using AAA17777 (Actin cardiac actin-Specific antibody) at dilution of 1:100)

product-image-AAA17777_IHC.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human heart using AAA17777 (Actin cardiac actin-Specific antibody) at dilution of 1:100)
Related Product Information for anti-Alpha cardiac actin antibody
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alphaactin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
42 kDa

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Product Notes

The Alpha cardiac actin (Catalog #AAA17777) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti- Alpha cardiac actin-specific antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha cardiac actin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:2000-1:10000 IHC: 1:100-1:400 IF: 1:50-1:500. Researchers should empirically determine the suitability of the Alpha cardiac actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha cardiac actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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