WB (Western Blot)
(Detection of human and mouse beta Actin by western blot. Samples: Whole cell lysate (50 ug) from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
beta Actin Recombinant Antibody | betaActin recombinant antibody
Rabbit anti-beta Actin Recombinant Monoclonal Antibody [BLR057F]
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
beta Actin, Recombinant Antibody; Rabbit anti-beta Actin Recombinant Monoclonal Antibody [BLR057F]; Beta-actin; PS1TP5BP1; PS1TP5-binding protein 1; beta cytoskeletal actin; actin, cytoplasmic 1; I(2)-actin; BRWS1; Actin, cytoplasmic 1; actin beta; betaActin recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR057F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
10ug/ml (varies by lot)
Applicable Applications for betaActin recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse beta Actin by western blot. Samples: Whole cell lysate (50 ug) from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse beta Actin by western blot. Samples: Whole cell lysate (50 ug) from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of human beta Actin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:400. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human beta Actin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:400. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human beta Actin by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human beta Actin by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody (AAA213553 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for betaActin recombinant antibody
Cytoskeletal beta-actin is one of six highly conserved actin isoforms that is expressed ubiquitously in eukaryotic cells. Cytoskeletal beta-actin functions to maintain cell structure and plays a role in motility.
Product Categories/Family for betaActin recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
375
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
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Product Notes
The betaActin actb (Catalog #AAA213553) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-beta Actin Recombinant Monoclonal Antibody [BLR057F] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's beta Actin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the betaActin actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Actin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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