Mouse beta-Tubulin Monoclonal Antibody | anti-TUBB3 antibody

beta-Tubulin Monoclonal Antibody (5G3)

Cat. Num. AAA30795

• Gene Names: TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
• Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast, Fish, Bovine, Rice
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: beta-Tubulin, Antibody; beta-Tubulin Monoclonal Antibody (5G3); Tubulin beta-3 chain; TUBB3; TUBB4; Tubulin beta-4 chain; Tubulin beta-III; anti-TUBB3 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast, Fish, Bovine, Rice
• Clonality: Monoclonal
• Clone Number: 5G3
• Specificity: The antibody detects endogenous beta-tubulin protein.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TUBB3 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:5000; IHC: 1:200; IF: 1:200
• Immunogen: Synthetic Peptide of beta-Tubulin
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). ?-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IF (Immunofluorescence)

(IF analysis of Hela with ?-Tubulin Monoclonal Antibody(Left) and DAPI (Right) diluted at 1:100.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.?-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.?-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.?-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(IHC Staining of Human colon tissue. diluted at 1:200.)

Application Data

(Meng. Yujiao. et al. "Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells." International journal of molecular medicine 39.5 (2017): 1101-1110.)

Application Data

(Diao. Zhiqing. et al. "SIRT3 consolidates heterochromatin and counteracts senescence." Nucleic acids research 49.8 (2021): 4203-4219.)

Application Data

(Cheng. X.. Wang. J.. Liu. C. et al. Zinc transporter SLC39A13/ZIP13 facilitates the metastasis of human ovarian cancer cells via activating Src/FAK signaling pathway. J Exp Clin Cancer Res 40. 199 (2021).)

WB (Western Blot)

(Western blot analysis of A549 (1). Rat brain (2). Mouse brain (3). Chicken lung (4) and Rabbit testis (5). Sheep muscle (6). diluted at 1:5000.)

Related Product Information for anti-TUBB3 antibody

tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Product Categories/Family for anti-TUBB3 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 308235963
• NCBI GeneID: 10381
• NCBI Accession #: NP_001184110.1
• NCBI GenBank Nucleotide #: NM_001197181.1
• UniProt Accession #: Q13509; Q9BTZ0; Q9BW10
• Molecular Weight: 50,433 Da
• NCBI Official Full Name: tubulin beta-3 chain isoform 2
• NCBI Official Synonym Full Names: tubulin, beta 3 class III
• NCBI Official Symbol: TUBB3
• NCBI Official Synonym Symbols: CDCBM; TUBB4; beta-4; CFEOM3A
• NCBI Protein Information: tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin
• UniProt Protein Name: Tubulin beta-3 chain
• UniProt Gene Name: TUBB3
• UniProt Synonym Gene Names: TUBB4
• UniProt Entry Name: TBB3_HUMAN