WB (Western Blot)
(BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
Mouse anti-Human, Mouse BMI-1 Monoclonal Antibody | anti-BMI-1 antibody
BMI-1 antibody (mAb)(Clone AF27)
Gene Names
Bmi1; Bmi-1; Pcgf4; AW546694
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
BMI-1, Antibody; BMI-1 antibody (mAb)(Clone AF27); PCGF4; RNF51; FLVI2/BMI1; anti-BMI-1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AF27
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-BMI-1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
WB (Western Blot)
(BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
ChIP (Chromatin Immunoprecipitation)
(BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.)
ChIP (Chromatin Immunoprecipitation)
(BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.)
Related Product Information for anti-BMI-1 antibody
Short Description: Our BMI-1 antibody (mAb)(Clone AF27) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence, Immunohistochemistry and Western blot; it has been shown to react with Human and Mouse samples.
Background: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.
Background: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.
Product Categories/Family for anti-BMI-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37kDa
NCBI Official Full Name
polycomb complex protein BMI-1
NCBI Official Synonym Full Names
Bmi1 polycomb ring finger oncogene
NCBI Official Symbol
Bmi1
NCBI Official Synonym Symbols
Bmi-1; Pcgf4; AW546694
NCBI Protein Information
polycomb complex protein BMI-1
UniProt Protein Name
Polycomb complex protein BMI-1
UniProt Gene Name
Bmi1
UniProt Synonym Gene Names
Bmi-1; Pcgf4
UniProt Entry Name
BMI1_MOUSE
Similar Products
Product Notes
The BMI-1 bmi1 (Catalog #AAA59958) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMI-1 antibody (mAb)(Clone AF27) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMI-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the BMI-1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.