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Mouse Bordetella pertussis Monoclonal Antibody

Bordetella pertussis

Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A chromatography (>90%).
Synonyms
Bordetella pertussis, Antibody; Bordetella pertussis; Anti -Bordetella pertussis; anti-Bordetella pertussis antibody
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
5E222
Specificity
Recognizes LOS-A of Bordetella pertussis and B. bronchiseptica. Antibody is bactericidal for Bordetella pertussis only.
Purity/Purification
Affinity Purified
Purified by Protein A chromatography (>90%).
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Concentration
0.1mg/mL (varies by lot)
Applicable Applications for anti-Bordetella pertussis antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Immunogen
Bordetella pertussis
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Bordetella pertussis antibody
Bordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens. Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28kD), S2 (MW 23kD), S3 (MW 22kD), S4 (MW 11.7kD) and S5 (MW 9.3kD), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface.
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Product Notes

The Bordetella pertussis (Catalog #AAA14652) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bordetella pertussis can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA and Western Blot. Dilution: Immunofluorescence: 1:10-1:50 ELISA: 1:20-1:200. Researchers should empirically determine the suitability of the Bordetella pertussis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bordetella pertussis, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.