IP (Immunoprecipitation)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300))
Mouse anti-Human Caldesmon Monoclonal Antibody | anti-CALD1 antibody
Anti-Caldesmon Antibody
Gene Names
CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
Caldesmon, Antibody; Anti-Caldesmon Antibody; CAD; CDM; Caldesmon; anti-CALD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Specificity
Recognizes endogenous levels of Caldesmon protein.
Form/Format
Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
Applicable Applications for anti-CALD1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Cellular Location
Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P13505}. Cytoplasm, myofibril {ECO:0000250|UniProtKB:P13505}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:P13505}. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) {ECO:0000250|UniProtKB:P13505}
Tissue Location
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IP (Immunoprecipitation)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300))
IP (Immunoprecipitation)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300))
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IH (1/100 - 1/300)Immunohistochemical analysis of Caldesmon staining in human liomyoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IH (1/100 - 1/300)Immunohistochemical analysis of Caldesmon staining in human liomyoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/300)Immunohistochemical analysis of Caldesmon staining in human fibroid formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/300)Immunohistochemical analysis of Caldesmon staining in human fibroid formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-CALD1 antibody
Mouse monoclonal antibody to Caldesmon
KLH-conjugated synthetic peptide encompassing a sequence within human Caldesmon. The exact sequence is proprietary.
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
KLH-conjugated synthetic peptide encompassing a sequence within human Caldesmon. The exact sequence is proprietary.
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,231 Da
NCBI Official Full Name
caldesmon isoform 2
NCBI Official Synonym Full Names
caldesmon 1
NCBI Official Symbol
CALD1
NCBI Official Synonym Symbols
CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
NCBI Protein Information
caldesmon
UniProt Protein Name
Caldesmon
UniProt Gene Name
CALD1
UniProt Synonym Gene Names
CAD; CDM; CDM
UniProt Entry Name
CALD1_HUMAN
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Product Notes
The CALD1 cald1 (Catalog #AAA293399) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Caldesmon Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caldesmon can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CALD1 cald1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caldesmon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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