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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD163-Monospecific Mouse Monoclonal Antibody (M130/2162). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human CD163 Monoclonal Antibody | anti-CD163 antibody
CD163 (Monocyte & Macrophage Marker)
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified Ab with BSA and Azide at 200ug/ml
Synonyms
CD163, Antibody; CD163 (Monocyte & Macrophage Marker); CD163; CD163 antigen; Macrophage-associated antigen; M130; CD163 molecule; Hemoglobin scavenger receptor; MM130; Scavenger receptor cysteine rich type 1 protein M130; anti-CD163 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
M130/2162
Specificity
This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1116
Applicable Applications for anti-CD163 antibody
IHC (Immunohistochemistry), ELISA
Positive Control
HPBL cells. Skin, Lung, Placenta or Histiocytoma.
Immunogen
Recombinant fragment (around aa 43-196) of human CD163 (exact sequence is proprietary)
Cellular Localization
Cell Surface & Cytoplasmic
Hu-chromosome Location
12p13.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD163-Monospecific Mouse Monoclonal Antibody (M130/2162). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD163-Monospecific Mouse Monoclonal Antibody (M130/2162). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS_PAGE
(SDS-PAGE Analysis Purified CD163-Monospecific Mouse Monoclonal Antibody (M130/2162). Confirmation of Purity and Integrity of Antibody.)
SDS_PAGE
(SDS-PAGE Analysis Purified CD163-Monospecific Mouse Monoclonal Antibody (M130/2162). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with CD163-Monospecific Mouse Monoclonal Antibody (M130/2162).)
References
Sean K. Lau, et al. Am J Clin Pathol 2004;122:794-801.
NCBI and Uniprot Product Information
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Product Notes
The CD163 (Catalog #AAA215296) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD163 (Monocyte & Macrophage Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CD163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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