IF (Immunofluorescence)
(Dilution: IF IH (1/100 - 1/300)Immunohistochemical analysis of CD163 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Mouse anti-Human CD163 Monoclonal Antibody | anti-CD163 antibody
Anti-CD163 Antibody
Gene Names
CD163; M130; MM130
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
CD163, Antibody; Anti-CD163 Antibody; M130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163; anti-CD163 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
Recognizes endogenous levels of CD163 protein.
Form/Format
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
Applicable Applications for anti-CD163 antibody
IHC (Immunohistochemistry)
Cellular Location
[Soluble CD163]: Secreted
Tissue Location
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Dilution: IF IH (1/100 - 1/300)Immunohistochemical analysis of CD163 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IF (Immunofluorescence)
(Dilution: IF IH (1/100 - 1/300)Immunohistochemical analysis of CD163 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Dilution: IH: (1/100 - 1/300)Immunohistochemical analysis of CD163 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Dilution: IH: (1/100 - 1/300)Immunohistochemical analysis of CD163 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-CD163 antibody
Mouse monoclonal antibody to CD163
KLH-conjugated synthetic peptide encompassing a sequence within human CD163. The exact sequence is proprietary.
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH- dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
KLH-conjugated synthetic peptide encompassing a sequence within human CD163. The exact sequence is proprietary.
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH- dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125,451 Da
NCBI Official Full Name
scavenger receptor cysteine-rich type 1 protein M130 isoform a
NCBI Official Synonym Full Names
CD163 molecule
NCBI Official Symbol
CD163
NCBI Official Synonym Symbols
M130; MM130
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_HUMAN
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Product Notes
The CD163 cd163 (Catalog #AAA293405) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD163 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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