FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD163 antibody 2A10 (2A10/11) (AAA72277). Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 2A10 (2A10/11) (AAA72277-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit CD163 Antibody | anti-CD163 antibody
Anti-CD163 [2A10 (2A10/11)], Rabbit IgG
Reactivity
Pig (Sus scrofa)
Applications
ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD163; N/A; Anti-CD163 [2A10 (2A10/11)], Rabbit IgG; Anti-CD163 [2A10 (2A10/11)]; M130; sCD163; Scavenger receptor cysteine-rich type 1 protein M130; anti-CD163 antibody
Host
Rabbit
Reactivity
Pig (Sus scrofa)
Isotype
Rabbit IgG, Lambda
Clone Number
2A10 (2A10/11)
Specificity
This antibody recognizes and binds porcine CD163.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD163 antibody
ELISA, IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD163 antibody 2A10 (2A10/11) (AAA72277). Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 2A10 (2A10/11) (AAA72277-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD163 antibody 2A10 (2A10/11) (AAA72277). Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 2A10 (2A10/11) (AAA72277-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of pig peripheral blood monocytes with anti-CD163 (AAA72277) 2A10 (2A10/11) Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2A10 (2A10/11) (AAA72277-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72277-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of pig peripheral blood monocytes with anti-CD163 (AAA72277) 2A10 (2A10/11) Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2A10 (2A10/11) (AAA72277-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72277-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD163 antibody
This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).
Product Categories/Family for anti-CD163 antibody
References
Bullido et al. Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens (1997); 49(4):403-13. PMID:9151393
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,061 Da
NCBI Official Full Name
scavenger receptor cysteine-rich type 1 protein M130
NCBI Official Symbol
CD163
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_PIG
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Product Notes
The CD163 cd163 (Catalog #AAA72277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD163 [2A10 (2A10/11)], Rabbit IgG reacts with Pig (Sus scrofa) and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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