Mouse anti-Human CD163 Antibody | anti-CD163 antibody

Anti-Hu CD163 Biotin

Cat. Num. AAA129370

• Gene Names: CD163; M130; MM130
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Synonyms: CD163; N/A; Anti-Hu CD163 Biotin; CD163 molecule; MM130, SCARI1; anti-CD163 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: GHI/61
• Specificity: The mouse monoclonal antibody GHI/61 recognizes an extracellular epitope CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
• Purity/Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD163 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot)
• Immunogen: Hairy cell leukemia cells
• Conjugation: Biotin
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD163 (GHI/61) Biotin antibody (concentration in sample 5ug/ml, Streptavidin APC).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD163 (GHI/61) Biotin antibody (concentration in sample 5ug/ml, Streptavidin APC).)

Related Product Information for anti-CD163 antibody

CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.

References

*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Bluml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95., URL: http://www.ncbi.nlm.nih.gov/pubmed/1592433 ,*Kusi KA, Gyan BA, Goka BQ, Dodoo D, Obeng-Adjei G, Troye-Blomberg M, Akanmori BD, Adjimani JP: Levels of soluble CD163 and severity of malaria in children in Ghana. Clin Vaccine Immunol. 2008 Sep;15(9):1456-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/18632918 ,*Bover LC, Cardó-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R: A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J Immunol. 2007 Jun 15;178(12):8183-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/17548657 ,*Moniuszko M, Kowal K, Rusák M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun;13(6):704-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/16760331,*Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K: CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol. 2006 Mar;168(3):822-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/16507898,*Philippidis P, Mason JC, Evans BJ, Nadra I, Taylor KM, Haskard DO, Landis RC: Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res. 2004 Jan 9;94(1):119-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/14656926,*Madsen M, Møller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg T, Moestrup SK: Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region. J Biol Chem. 2004 Dec 3;279(49):51561-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/15448162,*Møller HJ, Peterslund NA, Graversen JH, Moestrup SK: Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 2002 Jan 1;99(1):378-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/11756196,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993 Sep;23(9):2320-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/8370408,*Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/1592433,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Bjorklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Borner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,

NCBI and Uniprot Product Information

• NCBI GI #: 344179110
• NCBI GeneID: 9332
• NCBI Accession #: NP_004235.4
• NCBI GenBank Nucleotide #: NM_004244.5
• UniProt Accession #: Q86VB7; Q07898; Q07899; Q07900; Q07901; Q2VLH7; C9JIG2
• Molecular Weight: 125,451 Da
• NCBI Official Full Name: scavenger receptor cysteine-rich type 1 protein M130 isoform a
• NCBI Official Synonym Full Names: CD163 molecule
• NCBI Official Symbol: CD163
• NCBI Official Synonym Symbols: M130; MM130
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• UniProt Gene Name: CD163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_HUMAN