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Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Mouse CD178 Monoclonal Antibody | anti-CD178 antibody
MOUSE ANTI HUMAN CD178
Gene Names
FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA
Synonyms
CD178, Antibody; MOUSE ANTI HUMAN CD178; anti-CD178 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F2
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
281
Applicable Applications for anti-CD178 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Buffer Solution
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Application Data
(Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Application Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178)
Related Product Information for anti-CD178 antibody
Mouse anti Human CD178 antibody, clone 10F2 recognizes the human CD178 cell surface antigen, a 40kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis. Clone 10F2 is reported to block the activity of Fas ligand (Legembre et al. 2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,006 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 6 isoform 1
NCBI Official Synonym Full Names
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol
FASLG
NCBI Official Synonym Symbols
APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
NCBI Protein Information
tumor necrosis factor ligand superfamily member 6; CD95 ligand; apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; fas antigen ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 6
UniProt Gene Name
FASLG
UniProt Synonym Gene Names
APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
UniProt Entry Name
TNFL6_HUMAN
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Product Notes
The CD178 faslg (Catalog #AAA49370) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD178 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD178 faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD178, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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