FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA279306MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD276 recombinant antibody
CD276 Recombinant Monoclonal Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human, Cynomolgus
Applications
ELISA
Purity
Affinity-chromatography
Synonyms
CD276, Recombinant Antibody; CD276 Recombinant Monoclonal Antibody; 4Ig B7 H3 antibody; 4Ig-B7-H3 antibody; AU016588 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; CD_antigen=CD276 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; CD276_HUMAN antibody; Costimulatory molecule antibody; Flags: Precursor antibody; PSEC0249 antibody; UNQ309/PRO352 antibody$; CD276 recombinant antibody
Host
HEK293F Cell
Reactivity
Human, Cynomolgus
Clonality
Monoclonal
Isotype
hIgG1
Clone Number
5F9
Purity/Purification
Affinity-chromatography
Form/Format
Liquid; Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Applicable Applications for CD276 recombinant antibody
ELISA
Antigen Species
Human
Antigen
Recombinant Human CD276 protein
Conjugation
Non-conjugated
Research Area
Cancer, Immunology, Stem cells
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA279306MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA279306MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ELISA
(The Binding Activity of Human CD276 with Anti-CD276 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP733578HU(F2)) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.)
ELISA
(The Binding Activity of Human CD276 with Anti-CD276 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP733578HU(F2)) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.)
ELISA
(The Binding Activity of Human CD276 with Anti-CD276 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.)
ELISA
(The Binding Activity of Human CD276 with Anti-CD276 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,235 Da
NCBI Official Full Name
B7-H3 protein
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
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Product Notes
The CD276 cd276 (Catalog #AAA279306) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The CD276 Recombinant Monoclonal Antibody reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's CD276 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD276, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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