Mouse anti-Human CD3e (T-Cell Marker) Monoclonal Antibody | anti-CD3e antibody

CD3e (T-Cell Marker) Mouse Monoclonal Antibody [Clone C3e/1308]

Cat. Num. AAA214474

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD3e (T-Cell Marker), Antibody; CD3e (T-Cell Marker) Mouse Monoclonal Antibody [Clone C3e/1308]; CD 3E; CD3 epsilon; CD3 TCR complex; CD3E; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex; anti-CD3e antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: C3e/1308
• Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Form/Format: 200ug/ml of Ab purified from rabbit anti-serum by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

• Applicable Applications for anti-CD3e antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant human CD3e fragment (aa 23-119)
• Human Chromosome Location: 11q23.3
• Cellular Localization: Cell surface and cytoplasmic
• Positive Control: Jurkat cells. Tonsil and lymph node
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of Jurkat cells using CD3e Monoclonal Antibody (C3e/1308) followed by Goat anti-mouse IgG-CF488(Blue); Isotype Control (Red).)

WB (Western Blot)

(Western Blot Analysis of human Jurkat cell lysate using CD3e Monoclonal Antibody (C3e/1308).)

SDS-PAGE

(SDS-PAGE Analysis Purified CD3 epsilon Monoclonal Antibody (C3e/1308). Confirmation of Integrity and Purity of Antibody.)

WB (Western Blot)

(Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308))

References

Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

NCBI and Uniprot Product Information

• NCBI GeneID: 916
• UniProt Accession #: P07766
• Molecular Weight: 20kDa