FCM/FACS (Flow Cytometry)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.06 ug of Rat IgM kappa Isotype Control (FITC) (left) or 0.06 ug of CD43 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Rat CD43 Monoclonal Antibody | anti-CD43 antibody
CD43 antibody (FITC)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD43, Antibody; CD43 antibody (FITC); Monoclonal CD43; Anti-CD43; CD-43; CD 43; Ly-48; Sialophorin; Leukosialin; LSN; SPN; CD43; anti-CD43 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
R2/60
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
5 ul (2 ug)/test (varies by lot)
Applicable Applications for anti-CD43 antibody
FCM/FACS (Flow Cytometry)
Biological Significance
The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.
Tag/Conjugate
FITC
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
FCM/FACS (Flow Cytometry)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.06 ug of Rat IgM kappa Isotype Control (FITC) (left) or 0.06 ug of CD43 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
FCM/FACS (Flow Cytometry)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.06 ug of Rat IgM kappa Isotype Control (FITC) (left) or 0.06 ug of CD43 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD43 antibody
Rat monoclonal CD43 antibody (FITC)
Product Categories/Family for anti-CD43 antibody
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Product Notes
The CD43 (Catalog #AAA107049) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD43 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD43, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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