IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD43antibodyOX-58(AAA72484). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-58(AAA72484)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72484,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
CD43 recombinant antibody
Anti-CD43 [OX-58], Rabbit IgG, kappa
Gene Names
Spn; Lsn; Cd43; Lsn1; gpL115
Reactivity
Rat
Applications
immunoassay, Radioimmunoassay, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein A affinity purified
Synonyms
CD43, Recombinant Antibody; Anti-CD43 [OX-58], Rabbit IgG, kappa; CD43; Anti-CD43 [OX-58]; Leukosialin; Leukocyte sialoglycoprotein; Sialophorin; W3/13 antigen; OX 58; MRC OX 58; CD43 recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-58
Specificity
This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD43 recombinant antibody
RIA (Radioimmunoassay), FCM/FACS (Flow Cytometry), WB (Western Blot)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD43antibodyOX-58(AAA72484). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-58(AAA72484)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72484,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD43antibodyOX-58(AAA72484). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-58(AAA72484)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72484,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD43antibodyOX-58(AAA72484). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-58(AAA72484,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD43antibodyOX-58(AAA72484). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-58(AAA72484,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for CD43 recombinant antibody
This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.
References
Cyster et al. The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation. EMBO J. 1991 Apr;10(4):893-902. N/A1706994
Note on publication:
Describes the original use of this antibody, along with other anti-CD43 MAbs, in FC, WB and plate binding assay studies to determine the dimensions of the extracellular part of the leukosialin molecule, and to establish that the antigenic determinants recognized are largely based on linear protein sequences.
Note on publication:
Describes the original use of this antibody, along with other anti-CD43 MAbs, in FC, WB and plate binding assay studies to determine the dimensions of the extracellular part of the leukosialin molecule, and to establish that the antigenic determinants recognized are largely based on linear protein sequences.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,426 Da
NCBI Official Full Name
Leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
Spn
NCBI Official Synonym Symbols
Lsn; Cd43; Lsn1; gpL115
NCBI Protein Information
leukosialin; W3/13 antigen; leukocyte sialoglycoprotein; leukosianin
UniProt Protein Name
Leukosialin
UniProt Gene Name
Spn
UniProt Entry Name
LEUK_RAT
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Product Notes
The CD43 spn (Catalog #AAA72484) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD43 [OX-58], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD43 can be used in a range of immunoassay formats including, but not limited to, RIA (Radioimmunoassay), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD43 spn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD43, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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