FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD45RBantibodyOX-33(AAA72455) Ratsplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)orthemouseIgG1versionofOX-33(AAA72455-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-mouseIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
CD45RB recombinant antibody
Anti-CD45RB [OX-33], Rabbit IgG, kappa
Gene Names
Ptprc; Lca; RT7; CD45; L-CA; T200
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Protein A affinity purified
Synonyms
CD45RB, Recombinant Antibody; Anti-CD45RB [OX-33], Rabbit IgG, kappa; CD45RB; Anti-CD45RB [OX-33]; L-CA; LCA; OX33; CD45 restricted; MRC OX33; MRC OX-33; CD45 R; CD45RB recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-33
Specificity
This antibody binds CD45RB and enables B cell (no other lymphoid cells) labelling analyzed by FACS. In addition it can be used fr immunofluorescent staining with FC analysis, IP and IHC of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD45RB recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD45RBantibodyOX-33(AAA72455) Ratsplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)orthemouseIgG1versionofOX-33(AAA72455-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-mouseIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD45RBantibodyOX-33(AAA72455) Ratsplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)orthemouseIgG1versionofOX-33(AAA72455-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-mouseIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
WB (Western Blot)
(Western Blotusinganti-CD45RBantibodyOX-33(AAA72455) Ratspleenlysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotprobedwiththemouseIgG1versionofOX-33(AAA72455-1.1)at0.1ug/mlbeforedetectionusingananti-mousesecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-CD45RBantibodyOX-33(AAA72455) Ratspleenlysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotprobedwiththemouseIgG1versionofOX-33(AAA72455-1.1)at0.1ug/mlbeforedetectionusingananti-mousesecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
Related Product Information for CD45RB recombinant antibody
Binds only to high MWT CD45RB. CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant).
References
Kato et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med 17:6-49 1992 N/A1383383
Note on publication:
Describes the use of OX-78 in characterising CD48 as the ligand for CD2 in mice.
Note on publication:
Describes the use of OX-78 in characterising CD48 as the ligand for CD2 in mice.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,182 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 3
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc
NCBI Official Synonym Symbols
Lca; RT7; CD45; L-CA; T200
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
Ptprc
UniProt Synonym Gene Names
L-CA
UniProt Entry Name
PTPRC_RAT
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Product Notes
The CD45RB ptprc (Catalog #AAA72455) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD45RB [OX-33], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RB can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the CD45RB ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RB, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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