IP (Immunoprecipitation)
(mCD47 was immunoprecipitated using:Lane A:0.5 mg Mouse brain Whole Cell Lysate4 uL anti-mCD47 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCD47 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
CD47 recombinant antibody
Recombinant Anti-CD47 Antibody, Rabbit Monoclonal
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
CD47, Recombinant Antibody; Recombinant Anti-CD47 Antibody, Rabbit Monoclonal; CD47 molecule; Anti-9130415E20Rik Antibody; Anti-AA407862 Antibody; Anti-AI848868 Antibody; Anti-AW108519 Antibody; Anti-B430305P08Rik Antibody; Anti-IAP Antibody; Anti-Itgp Antibody; CD47 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
#050
Specificity
Mouse CD47
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for CD47 recombinant antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse CD47 Protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD47 (rM CD47).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(mCD47 was immunoprecipitated using:Lane A:0.5 mg Mouse brain Whole Cell Lysate4 uL anti-mCD47 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCD47 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
IP (Immunoprecipitation)
(mCD47 was immunoprecipitated using:Lane A:0.5 mg Mouse brain Whole Cell Lysate4 uL anti-mCD47 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCD47 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
WB (Western Blot)
(Anti-CD47 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDa)
WB (Western Blot)
(Anti-CD47 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDa)
Related Product Information for CD47 recombinant antibody
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
References
Brown EJ, et al. (2001) Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11(3): 130-5.
Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27.
Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.
Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27.
Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.
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Product Notes
The CD47 (Catalog #AAA259143) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD47 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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