Mouse CD5 Monoclonal Antibody | anti-CD5 antibody

CD5

Cat. Num. AAA62643

• Gene Names: CD5; T1; LEU1
• Reactivity: Human, Rhesus Monkey. Others not-tested.
• Applications: Immunoprecipitation, Functional Assay, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD5, Antibody; CD5; CD5 antigen (p56 62); LEU1; Ly12; LyA; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; T-cell surface glycoprotein CD5; anti-CD5 antibody

• Host: Mouse
• Reactivity: Human, Rhesus Monkey. Others not-tested.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: CRIS-1
• Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29. HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making of mature T-cell neoplasms.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 495

• Applicable Applications for anti-CD5 antibody: IP (Immunoprecipitation), FA (Functional Assay), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Localization: Cell surface
• Positive Control: 293T, Ramos, or MOLT-4 Cells. Tonsil.
• Human Chromosomal Location: 11q12.2
• Immunogen: Stimulated human leukocytes
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Non-hazardous . No MSDS required.

SDS-PAGE

Application Data

(FCM staining of human PBMCs using CD5 MAb (CRIS-1).)

References

1. Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
2. Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
3. Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992;148(5):1287-93.
4. Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996;5(1):167-9.

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NM_014207.3
• UniProt Accession #: P06127; A0N0P4; A8K9I3
• Molecular Weight: 67kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
• UniProt Protein Name: T-cell surface glycoprotein CD5
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN