FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC)
Mouse anti-Pig CD8 BETA CHAIN Monoclonal Antibody | anti-CD8 antibody
MOUSE ANTI PIG CD8 BETA CHAIN: FITC
Gene Names
CD8B; LY3; P37; LEU2; LYT3; CD8B1
Reactivity
Pig
Applications
Flow Cytometry, Functional Assay
Synonyms
CD8 BETA CHAIN, Antibody; MOUSE ANTI PIG CD8 BETA CHAIN: FITC; anti-CD8 antibody
Host
Mouse
Reactivity
Pig
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PPT23
Specificity
CD8 BETA
Form/Format
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
30
Applicable Applications for anti-CD8 antibody
FCM/FACS (Flow Cytometry)
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Max Ex/Em
Fluorophore : FITC
Excitation (nm): 490
Emission Max (nm): 525
Excitation (nm): 490
Emission Max (nm): 525
Immunogen
Porcine thymus membrane lysate
Fusion partners
Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line
Preparation and Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC)
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC)
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (This product))
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (This product))
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE)
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE)
Related Product Information for anti-CD8 antibody
Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.
Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,237 Da
NCBI Official Full Name
CD8 beta chain
NCBI Official Synonym Full Names
CD8b molecule
NCBI Official Symbol
CD8B
NCBI Official Synonym Symbols
LY3; P37; LEU2; LYT3; CD8B1
NCBI Protein Information
T-cell surface glycoprotein CD8 beta chain
UniProt Protein Name
T-cell surface glycoprotein CD8 beta chain
UniProt Gene Name
CD8B
UniProt Synonym Gene Names
CD8B1
UniProt Entry Name
CD8B_HUMAN
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Product Notes
The CD8 cd8b (Catalog #AAA50267) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG CD8 BETA CHAIN: FITC reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 BETA CHAIN can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD8 cd8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 BETA CHAIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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