Mouse CD80 Monoclonal Antibody | anti-CD80 antibody

MOUSE ANTI HUMAN CD80:FITC

Cat. Num. AAA49082

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD80, Antibody; MOUSE ANTI HUMAN CD80:FITC; anti-CD80 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-233
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-CD80 antibody: FCM/FACS (Flow Cytometry)
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Staining of Daudi B cells with Mouse anti Human CD80:Alexa Fluor 647)

Application Data

(Staining of Daudi B cells with Mouse anti Human CD80:FITC)

Related Product Information for anti-CD80 antibody

Mouse anti Human CD80 antibody, clone MEM-233 recognizes human CD80, also known as B7-1, a ~60kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells (Munro et al. 1994) and activated B-cells (Ranheim et al. 1993) CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33kDa, the presence of multiple (8) potential N-glycosylation sites (Chen et al. 1998) results in a migration corresponding to ~60kDa. Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 (Azuma et al. 1993) involved in T cell activation and proliferation (Vasu et al. 2003). Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics (van der Merwe et al. 1997). Site mutagenesis studies indicate residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it's receptors CTLA-4 and CD28 (Peach et al. 1995). Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching studies (Vasu et al. 2003). Functional studies using Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbach et al. 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.3
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80; B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CTLA-4 counter-receptor B7.1; activation B7-1 antigen; costimulatory factor CD80; costimulatory molecule variant IgV-CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7
• UniProt Entry Name: CD80_HUMAN