FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD81 antibody 1.3.3.22 (AAA72168). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody or the rabbit IgG version of 1.3.3.22 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit CD81 Monoclonal Antibody | anti-CD81 antibody
Anti-CD81 [1.3.3.22]
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD81, Antibody; Anti-CD81 [1.3.3.22]; TSPAN-28; Tetraspanin-28; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; TAPA-1; TAPA1; anti-CD81 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
1.3.3.22
Specificity
1.3.3.22 antibody recognizes CD81. Human CD81 is a member of the tetraspanin family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. It is present on a wide variety of leukocytes.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD81 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
11080483
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD81 antibody 1.3.3.22 (AAA72168). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody or the rabbit IgG version of 1.3.3.22 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD81 antibody 1.3.3.22 (AAA72168). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody or the rabbit IgG version of 1.3.3.22 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD81 antibody 1.3.3.22 (AAA72168) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD81 antibody 1.3.3.22 (AAA72168) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD81 antibody
Recombinant monoclonal antibody to CD81. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1.3.3.22.
This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
Product Categories/Family for anti-CD81 antibody
References
Allander et al. Hepatitis C virus envelope protein E2 binds to CD81 of tamarins. Virology. 2000 Nov 25;277(2):358-67.
Note on publication: This article describes the application of the 1.3.3.22 antibody used in a flow cytometry study.
Note on publication: This article describes the application of the 1.3.3.22 antibody used in a flow cytometry study.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,809 Da
NCBI Official Full Name
CD81 antigen
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
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Product Notes
The CD81 cd81 (Catalog #AAA72168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD81 [1.3.3.22] reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD81 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD81 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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