Application Data
(Fig.3: SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Rat anti-Human CD81 Monoclonal Antibody | anti-CD81 antibody
anti-human CD81 monoclonal antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CD81, Antibody; anti-human CD81 monoclonal antibody; TAPA-1; anti-CD81 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
QV-6A8-S3
Specificity
anti-human CD81 (TAPA-1)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
236
Applicable Applications for anti-CD81 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human CD81
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.3: SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.3: SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of Dubca cells using QV-6A8-S3 Cat. # GM-1113. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve).)
Application Data
(Fig.1: FACS analysis of Dubca cells using QV-6A8-S3 Cat. # GM-1113. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve).)
Related Product Information for anti-CD81 antibody
Background: CD81 (TAPA-1) belongs to the transmembrane 4 superfamily (tetraspanin family). CD81 is a widely expressed cell-surface protein that is characterized by the presence of four transmembranic domains, short N and C termini, a small extracellular loop (SEL) and a large extra-cellular loop (LEL) (1). It is expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin and plays a role in the regulation of cell development, cell-growth and signal transduction. CD81 plays a critical role in Hepatitis C Infection and is involved in HCV entry due to its ability to interact with virus' E1/E2 glycoproteins (2,3). CD81 is an essential HCV host factor as silencing of CD81 expression by CD81-specific monoclonal antibodies in hepatoma cells inhibits HCV entry (4).
References
Levy S, Todd SC and Maecker HT (1998). CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol 16:89-109.
Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G and Abrignana S (1998). Binding of Hepatitis C Virus to CD81. Science 282 no. 5390 pp. 938-941
Flint M, von Hahn T, Zhang J, Farquhar M, Jones CT, Balfe P, Rice CM and McKeating JA (2006). Diverse CD81 Proteins Support Hepatitis C Virus Infection. Journal of Virology p.11331- 11342
Fofana I, Xiao F, Thumann C, Turek M, Zona L, Tawar RG, Grunert F, Thompson J, Zeisel MB, Baumert TF (2013). A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission. PLoS One 21;8(5):e64221
Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G and Abrignana S (1998). Binding of Hepatitis C Virus to CD81. Science 282 no. 5390 pp. 938-941
Flint M, von Hahn T, Zhang J, Farquhar M, Jones CT, Balfe P, Rice CM and McKeating JA (2006). Diverse CD81 Proteins Support Hepatitis C Virus Infection. Journal of Virology p.11331- 11342
Fofana I, Xiao F, Thumann C, Turek M, Zona L, Tawar RG, Grunert F, Thompson J, Zeisel MB, Baumert TF (2013). A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission. PLoS One 21;8(5):e64221
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,809 Da
NCBI Official Full Name
CD81 antigen isoform 1
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen; tspan-28; tetraspanin-28; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1)
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
UniProt Entry Name
CD81_HUMAN
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Product Notes
The CD81 cd81 (Catalog #AAA71476) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD81 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD81 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD81 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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