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product-image-AAA312385_FACS8.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Rabbit CDC42 Monoclonal Antibody | anti-CDC42 antibody

CDC42 Antibody

Gene Names
CDC42; G25K; CDC42Hs
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
CDC42, Antibody; CDC42 Antibody; CDC42 antibody; CDC42_HUMAN antibody; CDC42Hs antibody; Cell division control protein 42 homolog antibody; Cell division cycle 42 (GTP binding protein 25kDa) antibody; Cell division cycle 42 antibody; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein; 25kD)) antibody; dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein; G25K antibody; G25K GTP-binding protein antibody; Growth regulating protein antibody; GTP binding protein 25kDa antibody; Small GTP binding protein CDC42 antibody; TKS antibody; anti-CDC42 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JJ086-04
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CDC42 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

product-image-AAA312385_FACS8.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

product-image-AAA312385_IHC10.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

product-image-AAA312385_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

product-image-AAA312385_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2)

product-image-AAA312385_WB15.jpg WB (Western Blot) (Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2)
Related Product Information for anti-CDC42 antibody
The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.
Product Categories/Family for anti-CDC42 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
998
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,259 Da
NCBI Official Full Name
cell division control protein 42 homolog isoform 1
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
G25K; CDC42Hs
NCBI Protein Information
cell division control protein 42 homolog; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A
UniProt Protein Name
Cell division control protein 42 homolog
UniProt Gene Name
CDC42
UniProt Entry Name
CDC42_HUMAN

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Product Notes

The CDC42 cdc42 (Catalog #AAA312385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC42 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC42 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDC42 cdc42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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