Rabbit anti-Human CDC42 Polyclonal Antibody | anti-CDC42 antibody

Anti-CDC42 Antibody, Rabbit Polyclonal

Cat. Num. AAA254292

• Gene Names: CDC42; G25K; CDC42Hs
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CDC42, Antibody; Anti-CDC42 Antibody, Rabbit Polyclonal; CDC42 Antibody, Rabbit PAb, Antigen Affinity Purified; cell division cycle 42; Anti-CDC42Hs Antibody; Anti-G25K Antibody; anti-CDC42 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CDC42
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDC42 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human CDC42 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CDC42 (rh CDC42). CDC42 specific IgG was purified by Human CDC42 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-CDC42 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(CDC42 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-CDC42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDC42 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)

Product Categories/Family for anti-CDC42 antibody

Non-Canonical Wnt Pathway

References

et al. Shinjo K, et al. (1990) Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci. 87(24):9853-7.Munemitsu S, et al. (1990) Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. Mol Cell Biol. 10(11):5977-82.Walker S J, et al. (2000) Activation of phospholipase D1 by Cdc42 requires the Rho insert region. J Biol Chem. 275(21):15665-8.Low B C, et al. (1999) Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42. J Biol Chem. 274(46): 33123-30.Zhang B, et al. (1998) Interaction of Rac1 with GTPase-activating proteins and putative effectors. A comparison with Cdc42 and RhoA. J Biol Chem. 273(15):8776-82.

NCBI and Uniprot Product Information

• NCBI GI #: 4757952
• NCBI GeneID: 998
• NCBI Accession #: NP_001782.1
• NCBI GenBank Nucleotide #: NM_001791.3
• UniProt Accession #: P60953; P21181; P25763; Q7L8R5; Q9UDI2
• Molecular Weight: 21,259 Da
• NCBI Official Full Name: cell division control protein 42 homolog isoform 1
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A
• UniProt Protein Name: Cell division control protein 42 homolog
• UniProt Gene Name: CDC42
• UniProt Entry Name: CDC42_HUMAN