Chk1 Recombinant Antibody | CHEK1 recombinant antibody

Phospho-Chk1 (Ser317) Antibody, Rabbit MAb

Cat. Num. AAA259507

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A
• Synonyms: Chk1, Recombinant Antibody; Phospho-Chk1 (Ser317) Antibody, Rabbit MAb; checkpoint kinase 1; Anti-CHK1 Antibody; Anti-OZEMA21 Antibody; CHEK1 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 0045
• Specificity: Human Phospho-Chk1 (Ser317)
• Purity/Purification: Protein A
• Form/Format: Liquid; 10 mM sodium HEPES, 150 mM NaCl, 100ug/mL BSA, 50% glycerol, pH 7.5

• Applicable Applications for CHEK1 recombinant antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic phosphopeptide corresponding to residues around Ser317 of human Chk1.
• Preparation: This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser317 of human Chk1.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Western blot analysis of extracts from serum-starved HEK-293, untreated (-)(line A); treated with UV (1 h; +)(line B); treated with UV and lambda-phosphatase (line C) using Phospho-Chk1 (Ser317) rabbit monoclonal Antibody (#AAA259507) at 1:2000 dilution.(Validation Experiment))

WB (Western Blot)

(Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with UV (1 h), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk1 (Ser317) rabbit monoclonal Antibody (#AAA259507) at 1:2000 dilution.(Validation Experiment))

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human testis, untreated (left) or lambda phosphatase-treated (right), using Phospho-Chk1 (Ser317) Antibody, Rabbit Monoclonal (#AAA259507) at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts from serum-starved HEK293, untreated (-) or treated with UV (1 h; +), using Phospho-Chk1 (Ser317) rabbit Monoclonal Antibody (#AAA259507) at 1:2000 dilution (upper) or Beta-Tubulin Loading Control Antibody, Rabbit MAb (Chimeric) at 1:40000 dilution (lower).)

Related Product Information for CHEK1 recombinant antibody

CHK1/CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1/CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1/CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1/CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1/CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Product Categories/Family for CHEK1 recombinant antibody

p53 Pathway; Cancer Drug Targets Tumor Suppressors

References

Che. P. e. al. (2000. Th. 1.. . crysta. structur. o. huma. cel. cycl. checkpoin. kinas. CHK1/CHEK1. Implication. fo. CHK1/CHEK. regulation. Cell. 10. (6). 681-92.
Sanche. Y. e. al. (1997. Conservatio. o. th. CHK1/CHEK. checkpoin. pathwa. i. mammals. linkag. o. DN. damag. t. Cd. regulatio. throug. Cdc25. Science. 27. (5331). 1497-501.
Flagg. G. e. al. (1998. Atm-dependen. interaction. o. . mammalia. CHK1/CHEK. homolo. wit. meioti. chromosomes. Cur. Biol. . (12). 977-86.
Chin. CC. e. al. (2005. Claspin. . regulato. o. CHK1/CHEK. i. DN. replicatio. stres. pathway. DN. Repair. . (8-9). 1033-7.