Mouse Cytokeratin Monoclonal Antibody | anti-KRT antibody

Cytokeratin, pan (Epithelial Marker)

Cat. Num. AAA215342

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human, Dog, Cat
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cytokeratin, Antibody; Cytokeratin, pan (Epithelial Marker); K1B; KRT1B; Keratin; type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39; anti-KRT antibody

• Host: Mouse
• Reactivity: Human, Dog, Cat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: PCK/3150
• Specificity: Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 279

• Applicable Applications for anti-KRT antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: Skin, adeno- or squamous carcinomas (IHC).
• Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: Multiple
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS_PAGE

(SDS-PAGE Analysis Purified Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nucleus stained with Reddot (Red).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).)

References

1. Gown AM, et al. Am J Clin Pathol. 1985;84:413. 2. Battifora H, Am J Surg Pathol. 1988;12:24

NCBI and Uniprot Product Information

• NCBI GI #: 1419564
• NCBI GeneID: 3856
• Molecular Weight: 40-67kDa (Multiple)
• NCBI Official Full Name: cytokeratin
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8