Mouse DNA Adenine Methylase Monoclonal Antibody | anti-dam antibody
DNA Adenine Methylase
Gene Names
dam; ECK3374; JW3350
Reactivity
Gram Negative Bacteria
Applications
Western Blot
Purity
Protein A/G Chromatography
Synonyms
DNA Adenine Methylase, Antibody; DNA Adenine Methylase; DAM; DNA adenine methyktransferase; Deoxyadenosyl-methyltransferase; anti-dam antibody
Host
Mouse
Reactivity
Gram Negative Bacteria
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RB1
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
278
Applicable Applications for anti-dam antibody
WB (Western Blot)
Immunogen
Recombinant DNA Adenine Methylase protein.
Source
Hybridoma obtained after immunization of mice with recombinant DNA Adenine Methylase protein.
Preparation and Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Related Product Information for anti-dam antibody
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. Antibody can be used for Western blotting (1-5 µg/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions. The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.
Product Categories/Family for anti-dam antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
DNA adenine methyltransferase
NCBI Official Symbol
dam
NCBI Official Synonym Symbols
ECK3374; JW3350
NCBI Protein Information
DNA adenine methyltransferase
UniProt Protein Name
DNA adenine methylase
UniProt Gene Name
dam
UniProt Entry Name
DMA_ECOLI
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Product Notes
The dam dam (Catalog #AAA60488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA Adenine Methylase reacts with Gram Negative Bacteria and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Adenine Methylase can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the dam dam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Adenine Methylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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