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product-image-AAA213622_WB8.jpg
WB (Western Blot)
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product-image-AAA213622_IP10.jpg
product-image-AAA213622_IHC11.jpg
product-image-AAA213622_IHC13.jpg
product-image-AAA213622_ICC15.jpg
product-image-AAA213622_WB8.jpg WB (Western Blot) (Detection of human Doublecortin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, SK-N-BE(2), HeLa, SK-N-SH, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

Doublecortin Recombinant Antibody | DCX recombinant antibody

Rabbit anti-Doublecortin Recombinant Monoclonal Antibody [BLR168J]

Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Doublecortin, Recombinant Antibody; Rabbit anti-Doublecortin Recombinant Monoclonal Antibody [BLR168J]; doublin; SCLH; neuronal migration protein doublecortin; lis-X; XLIS; doublecortex; DC; DBCN; LISX; lissencephalin-X; Neuronal migration protein doublecortin; doublecortin; DCX recombinant antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR168J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for DCX recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human Doublecortin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, SK-N-BE(2), HeLa, SK-N-SH, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA213622_WB8.jpg WB (Western Blot) (Detection of human Doublecortin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, SK-N-BE(2), HeLa, SK-N-SH, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human Doublecortin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1) used for IP at 20 ul/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin, AAA213622 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

product-image-AAA213622_IP10.jpg IP (Immunoprecipitation) (Detection of human Doublecortin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1) used for IP at 20 ul/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin, AAA213622 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemisry)

(Detection of mouse Doublecortin in FFPE juvenile mouse brain (dentate gyrus) by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

product-image-AAA213622_IHC11.jpg IHC (Immunohistochemisry) (Detection of mouse Doublecortin in FFPE juvenile mouse brain (dentate gyrus) by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohiostchemistry)

(Detection of human Doublecortin in FFPE astrocytoma by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

product-image-AAA213622_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human Doublecortin in FFPE astrocytoma by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human Doublecortin in FFPE SK-N-BE(2) by immunocytochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

product-image-AAA213622_ICC15.jpg ICC (Immunocytochemistry) (Detection of human Doublecortin in FFPE SK-N-BE(2) by immunocytochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody (AAA213622 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for DCX recombinant antibody
Doublecortin is a microtubule-associated protein required for the initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Doublecortin directs neuronal migration by regulating the organization and stability of microtubules. In addition, doublecortin interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43602].
Product Categories/Family for DCX recombinant antibody

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
UniProt Protein Name
Neuronal migration protein doublecortin
UniProt Gene Name
DCX
UniProt Synonym Gene Names
DBCN; LISX; Lis-X
UniProt Entry Name
DCX_HUMAN

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Product Notes

The DCX dcx (Catalog #AAA213622) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Doublecortin Recombinant Monoclonal Antibody [BLR168J] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Doublecortin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DCX dcx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Doublecortin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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