IHC (Immunohiostchemistry)
(Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Mouse anti-Human, Mouse EphA2 Monoclonal Antibody | anti-EphA2 antibody
EphA2 antibody
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
EphA2, Antibody; EphA2 antibody; Ephrin receptor EphA2; epoxide hydrolase 1 microsomal (xenobiotic);; anti-EphA2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
3D7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
976
Applicable Applications for anti-EphA2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human EphA2 (559-976aa) purified from E. coli
Clone Note
Anti-human EphA2 mAb, clone 3D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EphA2 protein.
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C
Avoid repeated freezing and thawing cycles.
IHC (Immunohiostchemistry)
(Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
IHC (Immunohiostchemistry)
(Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot)
(The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EphA2 extracellular domain transfected 293T cell lysate Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate)
WB (Western Blot)
(The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EphA2 extracellular domain transfected 293T cell lysate Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate)
Related Product Information for anti-EphA2 antibody
The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
Product Categories/Family for anti-EphA2 antibody
References
Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5 (3), 149-57; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59 (1), 58-67
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,305 Da
NCBI Official Full Name
ephrin type-A receptor 2 isoform 1
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
NCBI Protein Information
ephrin type-A receptor 2
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Gene Name
EPHA2
UniProt Synonym Gene Names
ECK
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Product Notes
The EphA2 epha2 (Catalog #AAA48031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EphA2 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EphA2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the EphA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphA2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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