Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power))
Mouse ESTROGEN RECEPTOR ALPHA Monoclonal Antibody | anti-ESR1 antibody
MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Synonyms
ESTROGEN RECEPTOR ALPHA, Antibody; MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA; anti-ESR1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F11
Form/Format
Tissue Culture Supernatant - lyophilised
Sequence Length
595
Applicable Applications for anti-ESR1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Reconstitution
Reconstitute with 1.0ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution.
Perservative Stabilisers
Perservative Stabilisers
Immunogen
Recombinant human estrogen receptor (alpha form)
Histology Positive Control Tissue
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power))
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power))
Application Data
(Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Application Data
(Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power))
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power))
Related Product Information for anti-ESR1 antibody
Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERalpha is a ~65kDa steroid hormone receptor containing an N-terminal AF-1).ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an AF-2 domain. ERalpha binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta (ERbeta) The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al. 2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargava et al. 2012). Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERalpha on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERalpha in breast cancer tissues (Ambroise et al. 2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,547 Da
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
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Product Notes
The ESR1 esr1 (Catalog #AAA49522) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ESTROGEN RECEPTOR ALPHA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESTROGEN RECEPTOR ALPHA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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