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WB (Western Blot)
(Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
FOXP3 recombinant antibody
Rabbit anti-FOXP3 Recombinant Monoclonal Antibody [BLR034F]
Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunofluorescence
Purity
Purified
Synonyms
FOXP3, Recombinant Antibody; Rabbit anti-FOXP3 Recombinant Monoclonal Antibody [BLR034F]; PIDX; scurfin; JM2; IPEX; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; FOXP3delta7; forkhead box protein P3; XPID; DIETER; AIID; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Forkhead box protein P3; forkhead box P3; FOXP3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR034F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for FOXP3 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Multiplex immunofluorescence (mIF)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
mIF: 1:250
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
mIF: 1:250
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human FOXP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2) used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody (AAA23804 lot 1). For blotting immunoprecipitated FOXP3, AAA23804 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human FOXP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2) used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody (AAA23804 lot 1). For blotting immunoprecipitated FOXP3, AAA23804 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human FOXP3 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human FOXP3 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human FOXP3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human FOXP3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human FOXP3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human FOXP3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human FOXP3 in FFPE MJ cells by immunocytochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human FOXP3 in FFPE MJ cells by immunocytochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody (AAA23804 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for FOXP3 recombinant antibody
FOXP3 is a transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. FOXP3 can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BZS1].
Product Categories/Family for FOXP3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,854 Da
NCBI Official Full Name
Homo sapiens ribosomal protein SA (RPSA), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ribosomal protein SA
NCBI Official Symbol
RPSA
NCBI Official Synonym Symbols
SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
NCBI Protein Information
40S ribosomal protein SA; 37 kDa laminin receptor; 37/67 kDa laminin receptor; colon carcinoma laminin-binding protein; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein precursor p40; multidrug resistance-associated protein MGr1-Ag
UniProt Protein Name
Forkhead box protein P3
UniProt Gene Name
FOXP3
UniProt Synonym Gene Names
IPEX
UniProt Entry Name
FOXP3_HUMAN
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Product Notes
The FOXP3 foxp3 (Catalog #AAA23804) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FOXP3 Recombinant Monoclonal Antibody [BLR034F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Multiplex immunofluorescence (mIF). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. mIF: 1:250. Researchers should empirically determine the suitability of the FOXP3 foxp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXP3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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