Application Data
(1. Exosomes extracted from Raji cells2. Exosomes extracted from U251 cells3. Raji cell Lysate)
Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody
Mouse Anti-GAPDH monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
IHC: 1:50-1:500
IF: 1:50-1:200
IP: 1ul-2ul
FC: 1:100-1:300
Application Data
(1. Exosomes extracted from Raji cells2. Exosomes extracted from U251 cells3. Raji cell Lysate)
Application Data
(1. Exosomes extracted from Raji cells2. Exosomes extracted from U251 cells3. Raji cell Lysate)
Application Data
(1.Exosomes extracted from MG63 cells2. Exosomes extracted from Ntera-2 cells3. MG63 cell Lysate)
Application Data
(1.Exosomes extracted from MG63 cells2. Exosomes extracted from Ntera-2 cells3. MG63 cell Lysate)
Application Data
(1.Exosomes extracted from HEPG2 cells2. Exosomes extracted from PC-3 cells3. Exosomes extracted from Hela cells4. Exosomes extracted from U87 cells5. Hela cell Lysate)
Application Data
(1.Exosomes extracted from HEPG2 cells2. Exosomes extracted from PC-3 cells3. Exosomes extracted from Hela cells4. Exosomes extracted from U87 cells5. Hela cell Lysate)
FCM (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with AAA18852 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with AAA18852 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA18852 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA18852 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of AAA18852 in Hela whole cell lysate.Lane 2: AAA18852 (1ml) + Hela whole cell lysate (500mg)Lane 3: Hela whole cell lysate (20mg)For western blotting, the blot was detected with AAA18852 at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of AAA18852 in Hela whole cell lysate.Lane 2: AAA18852 (1ml) + Hela whole cell lysate (500mg)Lane 3: Hela whole cell lysate (20mg)For western blotting, the blot was detected with AAA18852 at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cellswith AAA18852 at 1:220, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cellswith AAA18852 at 1:220, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA18852 at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA18852 at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistchemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA18852 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissueAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
WB (Western Blot)
(Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissueAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
WB (Western Blot)
(Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissueAll lanes GAPDH antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 1min)
WB (Western Blot)
(Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissueAll lanes GAPDH antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 1min)
WB (Western Blot)
(Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysateAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s)
WB (Western Blot)
(Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysateAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysateAll lanes: GAPDH antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysateAll lanes: GAPDH antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ugAll lanes: GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ugAll lanes: GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
WB (Western Blot)
(Positive WB detected in: 15ug hela whole cell lysateGAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
WB (Western Blot)
(Positive WB detected in: 15ug hela whole cell lysateGAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)
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Product Notes
The GAPDH (Catalog #AAA18852) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-GAPDH monoclonal Antibody reacts with Human, Rat, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC). WB: 1:5,000-1:1,600,000 IHC: 1:50-1:500 IF: 1:50-1:200 IP: 1ul-2ul FC: 1:100-1:300. Researchers should empirically determine the suitability of the GAPDH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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