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WB (Western Blot)
(Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.)
Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody
GAPDH Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
GAPDH, Antibody; GAPDH Antibody; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; 2699-; GAPD; anti-GAPDH antibody
Host
Mouse
Reactivity
Human, mouse, rat
Clonality
Monoclonal
Clone Number
1A10A10
Specificity
GAPDH recombinant protein is used to produce this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.44 (varies by lot)
Sequence Length
293
Applicable Applications for anti-GAPDH antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB~~1:2000~10000
IF~~1:25
IHC~~1:25
IF~~1:25
IHC~~1:25
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.)
WB (Western Blot)
(Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. AAA28634 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
WB (Western Blot)
(All lanes : Anti-GAPDH Antibody at 1:8000 dilutionLane 1: Hela whole cell lysatesLane 2: A549 whole cell lysatesLane 3: COS-7 whole cell lysatesLane 4: mouse brain lysatesLane 5: C6 whole cell lysatesLane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-GAPDH Antibody at 1:8000 dilutionLane 1: Hela whole cell lysatesLane 2: A549 whole cell lysatesLane 3: COS-7 whole cell lysatesLane 4: mouse brain lysatesLane 5: C6 whole cell lysatesLane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-GAPDH Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: C6 whole cell lysatesLane 3: Hela whole cell lysatesLane 4: HUVEC whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-GAPDH Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: C6 whole cell lysatesLane 3: Hela whole cell lysatesLane 4: HUVEC whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GAPDH antibody
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Product Categories/Family for anti-GAPDH antibody
References
Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity by antibodies present in the cerebrospinal fluid of patients with multiple sclerosis. Kölln J, et al. J Immunol, 2010 Aug 1. PMID 20610654. Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030. Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Suzuki A, et al. Cancer Genomics Proteomics, 2010 Jan-Feb. PMID 20181627. siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. Yego EC, et al. J Biol Chem, 2010 Jan 29. PMID 19940145.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36053
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA28634) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAPDH Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~1:2000~10000 IF~~1:25 IHC~~1:25. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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