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Application Data
(Wei. Yuzhen. et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.)
Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody
GAPDH (PTR2304) Monoclonal Antibody
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovin
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein G
Synonyms
GAPDH, Antibody; GAPDH (PTR2304) Monoclonal Antibody; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; anti-GAPDH antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovin
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
PTR2304
Specificity
This antibody detects endogenous levels of GAPDH protein.
Purity/Purification
Protein G
Form/Format
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applicable Applications for anti-GAPDH antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:10000-50000
IF: 1:100-500
ELISA: 1:50000-500000
IF: 1:100-500
ELISA: 1:50000-500000
Immunogen
Synthetic Peptide of GAPDH
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
Application Data
(Wei. Yuzhen. et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.)
Application Data
(Wei. Yuzhen. et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.)
Application Data
(Wang. Yingying. et al. "p75NTR?/? mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/??catenin pathway." Cell proliferation 53.4 (2020): e12800.)
Application Data
(Wang. Yingying. et al. "p75NTR?/? mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/??catenin pathway." Cell proliferation 53.4 (2020): e12800.)
Application Data
(Ho. Philip Wing-Lok. et al. "Age-dependent accumulation of oligomeric SNCA/?-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.)
Application Data
(Ho. Philip Wing-Lok. et al. "Age-dependent accumulation of oligomeric SNCA/?-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.)
Application Data
(Du. Shaobo. et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells againstultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).)
Application Data
(Du. Shaobo. et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells againstultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).)
Application Data
(Cheng. Xiaocheng. et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-?/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.)
Application Data
(Cheng. Xiaocheng. et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-?/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.)
Application Data
(Chen. Luchao. et al. "Effects of purified Omphalia lapidescens protein on metastasis. cell cycle. apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.)
Application Data
(Chen. Luchao. et al. "Effects of purified Omphalia lapidescens protein on metastasis. cell cycle. apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.)
WB (Western Blot)
(Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4 degree over night. secondary antibody)
WB (Western Blot)
(Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4 degree over night. secondary antibody)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Related Product Information for anti-GAPDH antibody
glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
Product Categories/Family for anti-GAPDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,053 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
Similar Products
Product Notes
The GAPDH gapdh (Catalog #AAA30794) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAPDH (PTR2304) Monoclonal Antibody reacts with Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovin and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P). WB: 1:10000-50000 IF: 1:100-500 ELISA: 1:50000-500000. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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