IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GCK protein in paraffin embedded Adenocarcinoma of Human breast tissue using GCK antibody)
Mouse GCK Monoclonal Antibody | anti-GCK antibody
GCK antibody
Gene Names
GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
GCK antibody was purified by affinity chromatography.
Synonyms
GCK, Antibody; GCK antibody; Monoclonal GCK; Anti-GCK; MAP4K2; Mitogen-Activated Protein Kinase 2; BL44; RAB8IP; anti-GCK antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3 E3
Purity/Purification
GCK antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
464
Applicable Applications for anti-GCK antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
GCK is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germin al centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
Cross-Reactivity
Human
Immunogen
GCK antibody was raised in mouse using a protein expressed in 293T cell transfected with human GCK expression vector as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GCK protein in paraffin embedded Adenocarcinoma of Human breast tissue using GCK antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GCK protein in paraffin embedded Adenocarcinoma of Human breast tissue using GCK antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GCK protein (Right) or empty vector (Left) detected with GCK antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GCK protein (Right) or empty vector (Left) detected with GCK antibody)
Related Product Information for anti-GCK antibody
Mouse monoclonal GCK antibody
Product Categories/Family for anti-GCK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,035 Da
NCBI Official Full Name
glucokinase isoform 3
NCBI Official Synonym Full Names
glucokinase (hexokinase 4)
NCBI Official Symbol
GCK
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
NCBI Protein Information
glucokinase
UniProt Protein Name
Glucokinase
UniProt Gene Name
GCK
UniProt Synonym Gene Names
HK IV; HK4
UniProt Entry Name
HXK4_HUMAN
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Product Notes
The GCK gck (Catalog #AAA74896) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GCK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the GCK gck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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