SDS-PAGE
(SDS-PAGE Analysis Purified GPX4/MCSP Mouse Monoclonal Antibody (LHM 2). Confirmation of Purity and Integrity of Antibody.)
Mouse GPX4/MCSP Monoclonal Antibody | anti-GPX4 antibody
GPX4/MCSP
Gene Names
GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
GPX4/MCSP, Antibody; GPX4/MCSP; Glutathione Peroxidase 4 (GPX4); Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP); Phospholipid hydroperoxidase (PHGPx); Sperm nucleus glutathione peroxidase (snGPx); anti-GPX4 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
LHM 2
Specificity
Glutathione Peroxidase 4 (GPX4) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with 1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
197
Applicable Applications for anti-GPX4 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
Positive Control
MCF7 or HepG2 cells. Human brain or malignant melanoma.
Immunogen
A 375P cells crude extract
Cellular Localization
Mitochondrion. Cytoplasm.
Hu-chromosome Location
19p13.3
Preparation and Storage
Antibody without azide: store at -20 to -80°C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified GPX4/MCSP Mouse Monoclonal Antibody (LHM 2). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified GPX4/MCSP Mouse Monoclonal Antibody (LHM 2). Confirmation of Purity and Integrity of Antibody.)
IF (Immunofluorescence)
(Confocal immunofluorescence image of HepG2 cells stained with GPX4/MCSP Mouse Monoclonal Antibody (LHM 2) followed by Goat anti-Mouse CF488 (green). Reddot is used to label the nuclei red.)
IF (Immunofluorescence)
(Confocal immunofluorescence image of HepG2 cells stained with GPX4/MCSP Mouse Monoclonal Antibody (LHM 2) followed by Goat anti-Mouse CF488 (green). Reddot is used to label the nuclei red.)
References
Kupsch, J.M., et al. 1995. Melanoma Res. 5: 403-411.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21kDa
NCBI Official Full Name
Phospholipid hydroperoxide glutathione peroxidase
NCBI Official Synonym Full Names
glutathione peroxidase 4
NCBI Official Symbol
GPX4
NCBI Official Synonym Symbols
MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
NCBI Protein Information
phospholipid hydroperoxide glutathione peroxidase
UniProt Protein Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Gene Name
GPX4
UniProt Synonym Gene Names
PHGPx; GPx-4; GSHPx-4
UniProt Entry Name
GPX4_HUMAN
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Product Notes
The GPX4 gpx4 (Catalog #AAA214995) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GPX4/MCSP reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPX4/MCSP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the GPX4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPX4/MCSP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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